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[应用于检测单纯疱疹病毒(HSV)的免疫技术的比较研究]

[Comparative study of immunologic techniques applied to the detection of herpes simplex virus (HSV)].

作者信息

Meguenni S, Bandoui D, Lahlou Y, El Kolli J, Benyakoub D, Bouguermouh A

出版信息

Arch Inst Pasteur Alger. 1989;57:25-38.

PMID:2562255
Abstract

Indirect immunofluorescence (IF), immunoperoxidase (IP) and seroneutralization tests were compared for detection and identification or Herpes simplex virus (HSV), directly in tissue smears or in cells inoculated with clinical materials. Detection and clear differentiation between the two HSV serotypes were obtained using rabbit immunoglobulin cross-absorbed with heterologous virus antigen. Immunofluorescent staining with specific monoclonal antibodies is as sensitive and more rapid than standard Vero cell cultures, for the laboratory diagnosis of HSV.

摘要

对间接免疫荧光法(IF)、免疫过氧化物酶法(IP)和血清中和试验进行了比较,以直接在组织涂片或接种临床材料的细胞中检测和鉴定单纯疱疹病毒(HSV)。使用用异源病毒抗原交叉吸收的兔免疫球蛋白可实现两种HSV血清型之间的检测和清晰区分。对于HSV的实验室诊断,用特异性单克隆抗体进行免疫荧光染色与标准的Vero细胞培养一样灵敏,且速度更快。

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