Lentfer T L, Pendl H, Gebhardt-Henrich S G, Fröhlich E K F, Von Borell E
a Swiss Federal Veterinary Office , Centre for Proper Housing: Poultry and Rabbits , Zollikofen , Switzerland.
Br Poult Sci. 2015 Apr;56(2):157-63. doi: 10.1080/00071668.2015.1008993.
Measuring the ratio of heterophils and lymphocytes (H/L) in response to different stressors is a standard tool for assessing long-term stress in laying hens but detailed information on the reliability of measurements, measurement techniques and methods, and absolute cell counts is often lacking. Laying hens offered different sites of the nest boxes at different ages were compared in a two-treatment crossover experiment to provide detailed information on the procedure for measuring and the difficulties in the interpretation of H/L ratios in commercial conditions. H/L ratios were pen-specific and depended on the age and aviary system. There was no effect for the position of the nest. Heterophiles and lymphocytes were not correlated within individuals. Absolute cell counts differed in the number of heterophiles and lymphocytes and H/L ratios, whereas absolute leucocyte counts between individuals were similar. The reliability of the method using relative cell counts was good, yielding a correlation coefficient between double counts of r > 0.9. It was concluded that population-based reference values may not be sensitive enough to detect individual stress reactions and that the H/L ratio as an indicator of stress under commercial conditions may not be useful because of confounding factors and that other, non-invasive, measurements should be adopted.
测量异嗜性粒细胞与淋巴细胞的比例（H/L）以应对不同应激源，是评估蛋鸡长期应激的标准工具，但关于测量可靠性、测量技术与方法以及绝对细胞计数的详细信息往往缺失。在一项双处理交叉实验中，对不同年龄提供不同产蛋箱位置的蛋鸡进行比较，以提供关于测量程序以及商业条件下H/L比例解读困难的详细信息。H/L比例因鸡舍而异，且取决于年龄和禽舍系统。产蛋箱位置没有影响。个体内异嗜性粒细胞和淋巴细胞不相关。异嗜性粒细胞和淋巴细胞的数量以及H/L比例的绝对细胞计数不同，而个体间的绝对白细胞计数相似。使用相对细胞计数的方法可靠性良好，两次计数之间的相关系数r > 0.9。得出的结论是，基于群体的参考值可能不够灵敏，无法检测个体应激反应，并且在商业条件下，H/L比例作为应激指标可能因混杂因素而无用，应采用其他非侵入性测量方法。
