Cai Zhibiao, Zeng Weijun, Tao Kai, Lu Fangfang, Gao Guodong, Yang Qian
Department of Neurosurgery, Tangdu Hospital, The Fourth Military Medical University, 569 Xinsi Road, Xi'an, Shaanxi 710038, China.
Department of Neurosurgery, Tangdu Hospital, The Fourth Military Medical University, 569 Xinsi Road, Xi'an, Shaanxi 710038, China.
Biochem Biophys Res Commun. 2015 Mar 6;458(2):227-33. doi: 10.1016/j.bbrc.2015.01.060. Epub 2015 Jan 24.
Oxidative stress and mitochondrial dysfunction have been linked to Parkinson's disease. DJ-1 is a recessive familial PD gene involved in antioxidative function and mitochondrial maintenance. Myricitrin, a flavanoid isolated from the root bark of Myrica cerifera, has potent antioxidative properties. In the present study, we investigated the protective effects of myricitrin against MPP(+)-induced mitochondrial dysfunction in SN4741 cells and attempted to elucidate the mechanisms underlying this protection. The results showed that incubating SN4741 cells with myricitrin significantly reduced cell death induced by the neurotoxin MPP(+). Furthermore, myricitrin protected cells from MPP(+)-induced effects on mitochondrial morphology and function. However, these protective effects were lost under DJ-1-deficient conditions. Thus, our results suggest that myricitrin alleviates MPP(+)-induced mitochondrial dysfunction and increases cell viability via DJ-1, indicating that myricitrin is a potential beneficial agent for age-related neurodegenerative diseases, particularly Parkinson's disease.
氧化应激和线粒体功能障碍与帕金森病有关。DJ-1是一种隐性家族性帕金森病基因，参与抗氧化功能和线粒体维持。杨梅素是从杨梅根皮中分离出的一种黄酮类化合物，具有强大的抗氧化特性。在本研究中，我们研究了杨梅素对MPP(+)诱导的SN4741细胞线粒体功能障碍的保护作用，并试图阐明这种保护作用的潜在机制。结果表明，用杨梅素处理SN4741细胞可显著减少神经毒素MPP(+)诱导的细胞死亡。此外，杨梅素保护细胞免受MPP(+)对线粒体形态和功能的影响。然而，在DJ-1缺陷的条件下，这些保护作用消失了。因此，我们的结果表明，杨梅素通过DJ-1减轻MPP(+)诱导的线粒体功能障碍并增加细胞活力，这表明杨梅素是一种对年龄相关神经退行性疾病，特别是帕金森病具有潜在益处的药物。
