壳聚糖固定化黑曲霉β-葡萄糖苷酶
Immobilization of Aspergillus beta-glucosidase on chitosan.
作者信息
Bissett F, Sternberg D
出版信息
Appl Environ Microbiol. 1978 Apr;35(4):750-5. doi: 10.1128/aem.35.4.750-755.1978.
Abstract
beta-Glucosidase of Aspergillus phoenicis QM 329 was immobilized on chitosan, using the bifunctional agent glutaraldehyde. The most active preparation based on the amount of support contained a 1:2.5 enzyme-to-chitosan ratio (wt/wt). However, the specific activity of the bound enzyme decreased from 10 to 1% with increasing enzyme-to-chitosan ratio. Compared with free beta-glucosidase, the immobilized enzyme exhibited: (i) a similar pH optimum but more activity at lower pH values; (ii) improved thermal stability; (iii) a similar response to inhibition by glucose; and (iv) mass transfer limitations as reflected by higher apparent Km and lower energy of activation.
摘要
使用双功能试剂戊二醛将嗜热栖热放线菌QM 329的β-葡萄糖苷酶固定在壳聚糖上。基于载体用量，活性最高的制剂中酶与壳聚糖的比例为1:2.5（重量/重量）。然而，随着酶与壳聚糖比例的增加，结合酶的比活性从10%降至1%。与游离β-葡萄糖苷酶相比，固定化酶表现出：（i）类似的最适pH，但在较低pH值下活性更高；（ii）热稳定性提高；（iii）对葡萄糖抑制的反应类似；以及（iv）传质限制，表现为较高的表观Km和较低的活化能。
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