壳聚糖固定化黑曲霉β-葡萄糖苷酶
Immobilization of Aspergillus beta-glucosidase on chitosan.
作者信息
Bissett F, Sternberg D
出版信息
Appl Environ Microbiol. 1978 Apr;35(4):750-5. doi: 10.1128/aem.35.4.750-755.1978.
Abstract
beta-Glucosidase of Aspergillus phoenicis QM 329 was immobilized on chitosan, using the bifunctional agent glutaraldehyde. The most active preparation based on the amount of support contained a 1:2.5 enzyme-to-chitosan ratio (wt/wt). However, the specific activity of the bound enzyme decreased from 10 to 1% with increasing enzyme-to-chitosan ratio. Compared with free beta-glucosidase, the immobilized enzyme exhibited: (i) a similar pH optimum but more activity at lower pH values; (ii) improved thermal stability; (iii) a similar response to inhibition by glucose; and (iv) mass transfer limitations as reflected by higher apparent Km and lower energy of activation.
摘要
使用双功能试剂戊二醛将嗜热栖热放线菌QM 329的β-葡萄糖苷酶固定在壳聚糖上。基于载体用量，活性最高的制剂中酶与壳聚糖的比例为1:2.5（重量/重量）。然而，随着酶与壳聚糖比例的增加，结合酶的比活性从10%降至1%。与游离β-葡萄糖苷酶相比，固定化酶表现出：（i）类似的最适pH，但在较低pH值下活性更高；（ii）热稳定性提高；（iii）对葡萄糖抑制的反应类似；以及（iv）传质限制，表现为较高的表观Km和较低的活化能。
相似文献
1
Immobilization of Aspergillus beta-glucosidase on chitosan.壳聚糖固定化黑曲霉β-葡萄糖苷酶
Appl Environ Microbiol. 1978 Apr;35(4):750-5. doi: 10.1128/aem.35.4.750-755.1978.
2
Stabilization of dimeric β-glucosidase from Aspergillus niger via glutaraldehyde immobilization under different conditions.不同条件下戊二醛固定化黑曲霉二聚体β-葡萄糖苷酶的稳定性。
Enzyme Microb Technol. 2018 Mar;110:38-45. doi: 10.1016/j.enzmictec.2017.12.007. Epub 2017 Dec 22.
3
Immobilization of β-glucosidase on a magnetic nanoparticle improves thermostability: application in cellobiose hydrolysis.β-葡萄糖苷酶固定在磁性纳米颗粒上提高了热稳定性：在纤维二糖水解中的应用。
Bioresour Technol. 2013 May;135:2-6. doi: 10.1016/j.biortech.2013.01.047. Epub 2013 Jan 23.
4
Physiochemical and Thermodynamic Characterization of Highly Active Mutated Aspergillus niger β-glucosidase for Lignocellulose Hydrolysis.用于木质纤维素水解的高活性突变黑曲霉β-葡萄糖苷酶的物理化学和热力学表征
Protein Pept Lett. 2018;25(2):208-219. doi: 10.2174/0929866525666180130161504.
5
[Study on isoflavone active aglycone preparation by immobilized beta-glucosidase from Aspergillus niger].[黑曲霉固定化β-葡萄糖苷酶制备异黄酮活性苷元的研究]
Sheng Wu Gong Cheng Xue Bao. 2007 Nov;23(6):1060-4.
6
Beta-glucosidase multiplicity from Aspergillus tubingensis CBS 643.92: purification and characterization of four beta-glucosidases and their differentiation with respect to substrate specificity, glucose inhibition and acid tolerance.来自泡盛曲霉CBS 643.92的β-葡萄糖苷酶多样性：四种β-葡萄糖苷酶的纯化与表征及其在底物特异性、葡萄糖抑制和耐酸性方面的差异
Appl Microbiol Biotechnol. 2001 Mar;55(2):157-63. doi: 10.1007/s002530000462.
7
Fructo-oligosaccharides production by an Aspergillus aculeatus commercial enzyme preparation with fructosyltransferase activity covalently immobilized on FeO-chitosan-magnetic nanoparticles.固定化在 FeO-壳聚糖-磁性纳米粒子上的具转果糖基酶活性的 Aspergillus aculeatus 商业酶制剂生产果寡糖。
Int J Biol Macromol. 2020 May 1;150:922-929. doi: 10.1016/j.ijbiomac.2020.02.152. Epub 2020 Feb 15.
8
Catalytic properties of the immobilized Aspergillus tamarii xylanase.固定化黑曲霉木聚糖酶的催化特性。
Microbiol Res. 2002;157(4):275-81. doi: 10.1078/0944-5013-00165.
9
Preparation and activity of bubbling-immobilized cellobiase within chitosan-alginate composite.壳聚糖-海藻酸钠复合载体中鼓泡固定化纤维素酶的制备及活性。
Prep Biochem Biotechnol. 2010;40(1):57-64. doi: 10.1080/10826060903392939.
10
Flow kinetics of immobilized beta-glucosidase.
Biochem Cell Biol. 1986 Feb;64(2):139-45. doi: 10.1139/o86-022.
引用本文的文献
1
Highly porous core-shell chitosan beads with superb immobilization efficiency for 121 inulosucrase and production of inulin-type fructooligosaccharides.具有极高固定化效率的高孔隙率核壳型壳聚糖珠粒，用于固定菊粉蔗糖酶并生产菊粉型低聚果糖。
RSC Adv. 2018 May 9;8(30):17008-17016. doi: 10.1039/c8ra02241k. eCollection 2018 May 3.
2
Gestational diabetes exacerbates maternal immune activation effects in the developing brain.妊娠糖尿病加剧了母体免疫激活对发育中大脑的影响。
Mol Psychiatry. 2018 Sep;23(9):1920-1928. doi: 10.1038/mp.2017.191. Epub 2017 Sep 26.
3
Immobilization of Glycoside Hydrolase Families GH1, GH13, and GH70: State of the Art and Perspectives.糖苷水解酶家族GH1、GH13和GH70的固定化：现状与展望
Molecules. 2016 Aug 17;21(8):1074. doi: 10.3390/molecules21081074.
4
Trichoderma viride cellulase induces resistance to the antibiotic pore-forming peptide alamethicin associated with changes in the plasma membrane lipid composition of tobacco BY-2 cells.绿色木霉纤维素酶诱导烟草 BY-2 细胞产生对抗抗生素孔形成肽菌素的抗性，同时伴随细胞膜脂成分的变化。
BMC Plant Biol. 2010 Dec 14;10:274. doi: 10.1186/1471-2229-10-274.
5
Production and Characterization of Cellulase and beta-Glucosidase from a Mutant of Alternaria alternata.纤维素酶和β-葡萄糖苷酶的产生及特性研究，一株链格孢菌突变株。
Appl Environ Microbiol. 1984 Mar;47(3):560-5. doi: 10.1128/aem.47.3.560-565.1984.
6
Purification and Properties of beta-Glucosidase from Aspergillus terreus.黑曲霉β-葡萄糖苷酶的纯化和性质。
Appl Environ Microbiol. 1982 Dec;44(6):1289-95. doi: 10.1128/aem.44.6.1289-1295.1982.
7
Repression of endo-1,4-beta-glucanase formation in Penicillium janthinellum and product inhibition of its 1,4-beta-glucanases and cellobiases.黄绿青霉中内切-1,4-β-葡聚糖酶形成的抑制及其1,4-β-葡聚糖酶和纤维二糖酶的产物抑制
J Bacteriol. 1982 Feb;149(2):783-6. doi: 10.1128/jb.149.2.783-786.1982.
8
Purification and properties of a stable beta-glucosidase from an extremely thermophilic anaerobic bacterium.来自极端嗜热厌氧细菌的一种稳定β-葡萄糖苷酶的纯化及性质
Biochem J. 1987 May 1;243(3):779-87. doi: 10.1042/bj2430779.
本文引用的文献
1
Letter: Isolation and immobilization of beta-D-glucosidase from alcaligenes faecalis.
Biotechnol Bioeng. 1974 Oct;16(10):1413-8. doi: 10.1002/bit.260161010.
2
beta-Glucosidase: microbial production and effect on enzymatic hydrolysis of cellulose.β-葡萄糖苷酶：微生物生产及其对纤维素酶解的影响
Can J Microbiol. 1977 Feb;23(2):139-47. doi: 10.1139/m77-020.
3
Immobilized enzymes on chitosan columns: alpha-chymotrypsin and acid phosphatase.壳聚糖柱上固定化酶：α-胰凝乳蛋白酶和酸性磷酸酶。
Biotechnol Bioeng. 1976 Oct;18(10):1445-54. doi: 10.1002/bit.260181011.
4
Kinetics of the hydrolysis of cellulose by beta-1,4-glucan cellobiohydrolase of Trichoderma viride.绿色木霉β-1,4-葡聚糖纤维二糖水解酶水解纤维素的动力学
Can J Biochem. 1977 Jun;55(6):644-50. doi: 10.1139/o77-093.
5
Characteristics of yeast invertase immobilized on porous cellulose beads.固定在多孔纤维素珠上的酵母转化酶的特性
Biotechnol Bioeng. 1977 Mar;19(3):365-75. doi: 10.1002/bit.260190307.
Zotero 插件