College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an, Shaanxi 710062, PR China.
College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an, Shaanxi 710062, PR China.
Food Chem. 2015 Jun 1;176:7-11. doi: 10.1016/j.foodchem.2014.12.040. Epub 2014 Dec 18.
A simple and efficient HPLC fingerprint method was developed and validated for quality control of the polyphenols extracted from pomegranate peel (PPPs). Ten batches of pomegranate collected from different orchards in Shaanxi Lintong of China were used to establish the fingerprint. For the fingerprint analysis, 15 characteristic peaks were selected to evaluate the similarities of 10 batches of the PPPs. The similarities of the PPPs samples were all more than 0.968, indicating that the samples from different areas of Lintong were consistent. Additionally, simultaneous quantification of eight monophenols (including gallic acid, punicalagin, catechin, chlorogenic acid, caffeic acid, epicatechin, rutin, and ellagic acid) in the PPPs was conducted to interpret the consistency of the quality test. The results demonstrated that the HPLC fingerprint as a characteristic distinguishing method combining similarity evaluation and quantitative analysis can be successfully used to assess the quality and to identify the authenticity of the PPPs.
建立并验证了一种简单高效的 HPLC 指纹图谱法，用于控制从石榴皮中提取的多酚（PPPs）的质量。使用来自中国陕西临潼的 10 批石榴来建立指纹图谱。在指纹图谱分析中，选择了 15 个特征峰来评估 10 批 PPPs 的相似度。PPPs 样品的相似度均大于 0.968，表明来自临潼不同地区的样品是一致的。此外，还对 PPPs 中的 8 种单酚（包括没食子酸、安石榴甙、儿茶素、绿原酸、咖啡酸、表儿茶素、芦丁和鞣花酸）进行了同时定量分析，以解释质量测试的一致性。结果表明，HPLC 指纹图谱作为一种结合相似性评价和定量分析的特征鉴别方法，可成功用于评估 PPPs 的质量和鉴别其真伪。
