Ding Wenzheng, Wang Hanxue, Zhou Quan, Wu Chao, Gao Xiaoli, Cheng Xuemei, Tian Li, Wang Changhong
Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine; The MOE Key Laboratory for Standardization of Chinese Medicines; Shanghai R&D Centre for Standardization of Chinese Medicines, Shanghai, China.
Shanghai TCM-Integrated Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Biomed Chromatogr. 2019 Dec;33(12):e4690. doi: 10.1002/bmc.4690. Epub 2019 Sep 15.
Traditionally, pomegranate (Punica granatum L.) has been consumed as fresh fruit or as pomegranate juice. Pomegranate peel, the dried husk of P· granatum, is an important herbal medicine for treating diarrhea, hemostasis and insect-induced abdominal pain in China. However, the quality control methods for pomegranate peel remain unsatisfactory. In this work, a new HPLC-based qualitative and quantitative method for quality control of pomegranate peel was developed and validated for the simultaneous determination of polyphenols and triterpenes (including punicalagins A and B, ellagic acid, oleanolic acid and ursolic acid) by solvent extraction and ratio blending method in tandem with wavelength switching. The average recoveries were 98.07-100.61% with relative standard deviation no more than 4.27%. In addition, the fingerprint analysis was conducted to interpret the consistency of the quality test. Thirteen characteristic peaks were selected to evaluate the similarities of 16 batches of pomegranate peel. The similarities of samples were all more than 0.80, indicating that the samples from different areas of China were consistent. The results demonstrated that quantitative analysis and the HPLC fingerprint as a characteristic distinguishing method combining similarity evaluation can be successfully used to assess the quality and to identify the authenticity of pomegranate peel.
传统上，石榴（Punica granatum L.）被作为新鲜水果或石榴汁食用。石榴皮，即石榴（P·granatum）的干燥果皮，在中国是治疗腹泻、止血和虫积腹痛的一种重要草药。然而，石榴皮的质量控制方法仍不尽人意。在这项工作中，开发了一种基于高效液相色谱的石榴皮质量控制定性和定量新方法，并通过溶剂萃取和比例混合法结合波长切换，对多酚和三萜类化合物（包括石榴皮素A和B、鞣花酸、齐墩果酸和熊果酸）进行同时测定并验证。平均回收率为98.07 - 100.61%，相对标准偏差不超过4.27%。此外，进行了指纹图谱分析以阐释质量检测的一致性。选择13个特征峰来评估16批石榴皮的相似度。样品的相似度均大于0.80，表明来自中国不同地区的样品是一致的。结果表明，定量分析以及作为特征鉴别方法并结合相似度评价的高效液相色谱指纹图谱可成功用于评估石榴皮的质量并鉴定其真伪。
