College of Food Science, Woosuk University, Samrea-Up, Wanju-Kun, Jeonbuk Province 565-701, Republic of Korea.
Department of Applied Life Science, College of Life and Environment Science, Konkuk University, Seoul, Republic of Korea.
Food Chem. 2015 Jun 1;176:254-62. doi: 10.1016/j.foodchem.2014.12.073. Epub 2014 Dec 25.
A high resolution LC-ESI-TOF-MS analytical method was established for the rapid isolation, identification, and quantification of 12 isoflavones in soybean and soybean products. Individual isoflavones were identified on the basis of the accurate mass data of their respective protonated mass ions, Na or K adduct ions, fragment ions, and isotope ion patterns. The protonated mass ions of isoflavones were extracted for their quantification in soybean products. Twelve different isoflavones in the soybean products were fully separated, identified and quantified within 12min separation time. The established LC-TOF/MS was an effective analytical method for the simultaneous characterization and quantification of isoflavones with exceptionally short analytical time, high selectivity, a high linearity (r(2)>0.992) in a wide range, low LOD and LOQ, high precision, inter-and intra-day repeatability, and no significant matrix effect. Furthermore, it requires simple sample preparation procedure (solvent extraction, dilution, and syringe filtration).
建立了一种高分辨 LC-ESI-TOF-MS 分析方法，用于快速分离、鉴定和定量大豆及其制品中的 12 种异黄酮。根据各自质子化质量离子、Na 或 K 加合物离子、碎片离子和同位素离子模式的准确质量数据，鉴定了各个异黄酮。对异黄酮的质子化质量离子进行提取，用于定量分析大豆制品中的异黄酮。在 12min 的分离时间内，大豆制品中的 12 种不同异黄酮得到了完全分离、鉴定和定量。所建立的 LC-TOF/MS 是一种有效的分析方法，用于同时对异黄酮进行特征化和定量分析，具有分析时间极短、选择性高、在较宽范围内具有高线性（r(2)>0.992）、低检测限和定量限、高精确度、日内和日间重复性以及无显著基质效应的特点。此外，它还需要简单的样品制备程序（溶剂萃取、稀释和注射器过滤）。
