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基于多酚和蛋白质含量的豆类副产品在潜在营养保健应用中的价值提升。

Valorization of Legume By-Products Based on Polyphenols and Protein Contents for Potential Nutraceutical Applications.

作者信息

Terenzi Cristina, Bermudez Gabriela, Medri Francesca, Montanari Serena, Bucar Franz, Andrisano Vincenza

机构信息

Department for Life Quality Studies, University of Bologna, Corso D'Augusto 237, 47921 Rimini, Italy.

Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz, Beethovenstraße 8, 8010 Graz, Austria.

出版信息

Antioxidants (Basel). 2024 Dec 14;13(12):1531. doi: 10.3390/antiox13121531.

DOI:10.3390/antiox13121531
PMID:39765859
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11727440/
Abstract

A significant amount of agri-food by-products is generated by large food industry production lines. Aligned with the principles of a circular economy, this project aims to recycle and valorize legumes, such as beans, green beans and soy by-products characterized by different heat treatments, maturation stages and cultivation methods. The valorization of food waste involved the development of an Ultrasound-Assisted Extraction (UAE) method to isolate polyphenols. Analytical techniques, including UHPLC-DAD-ESI-MS, were used to identify polyphenols in legume, green bean and soy extracts obtained through UAE. Additionally, UV-Vis spectrophotometric assays measured the Total Phenolic Content (TPC) and Total Antioxidant Status (TAS), while the Kjeldahl method was employed to assess the protein content in each UAE extract. The analyses revealed a variety of valuable polyphenols in legume, green bean and soy by-products. For instance, bean by-products contain feruloyl glucaric acid derivatives, green beans by-products have different types of flavonols such as quercetin-3-O-glucuronide, and soy by-products are rich in isoflavones. These findings demonstrate the potential for formulating nutraceuticals from these by-products' extracts.

摘要

大型食品工业生产线会产生大量的农业食品副产品。本项目遵循循环经济原则,旨在回收利用并提升豆类(如菜豆、绿豆)以及大豆副产品的价值,这些副产品具有不同的热处理方式、成熟阶段和种植方法。食品废弃物的价值提升涉及到开发一种超声辅助萃取(UAE)方法来分离多酚。包括超高效液相色谱 - 二极管阵列 - 电喷雾电离质谱(UHPLC - DAD - ESI - MS)在内的分析技术,用于鉴定通过UAE获得的豆类、绿豆和大豆提取物中的多酚。此外,紫外 - 可见分光光度法测定总酚含量(TPC)和总抗氧化状态(TAS),同时采用凯氏定氮法评估每种UAE提取物中的蛋白质含量。分析结果表明,豆类、绿豆和大豆副产品中含有多种有价值的多酚。例如,菜豆副产品含有阿魏酰葡萄糖醛酸衍生物,绿豆副产品含有不同类型的黄酮醇,如槲皮素 - 3 - O - 葡萄糖醛酸苷,大豆副产品富含异黄酮。这些发现证明了从这些副产品提取物中制备营养保健品的潜力。

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