Hong Yan, He Zhuo-Jing, Tao Wei, Fu Ting, Wang Yan-Kun, Chen Yong
Yan Hong, Zhuo-Jing He, Wei Tao, Ting Fu, Yan-Kun Wang, Yong Chen, Institute of Bioengineering, Zhejiang Academy of Medical Science, Hangzhou 310013, Zhejiang Province, China.
World J Gastroenterol. 2015 Jan 21;21(3):862-7. doi: 10.3748/wjg.v21.i3.862.
To investigate whether Z:ZCLA Mongolian gerbils are readily susceptible to infection by human hepatitis E virus (HEV).
Z:ZCLA Mongolian gerbils were infected with a clinical HEV strain isolated from an acute hepatitis E patient, and virus pathogenesis was assessed in this host. Non-infected gerbils served as the control group. Feces samples from gerbils were collected weekly for reverse transcription-nested polymerase chain reaction. Serum anti-HEV IgG and alanine aminotransferase (ALT) were detected by enzyme linked immunosorbent assay. At sacrifice, each animal's liver, spleen and kidney were collected for histopathologic examination.
HEV-infected gerbils showed fatigue, with histopathological changes observed in the liver, spleen and kidney. HEV RNA was detected in fecal samples taken at day 7 after inoculation and the detectable levels lasted out to day 42 after inoculation. Interestingly, ALT levels were only moderately increased in the HEV-infected animals compared with the non-infected control group.
Z:ZCLA Mongolian gerbils are susceptible to human HEV.
研究Z:ZCLA蒙古沙鼠是否易于感染人戊型肝炎病毒（HEV）。
用从一名急性戊型肝炎患者分离出的临床HEV毒株感染Z:ZCLA蒙古沙鼠，并在该宿主中评估病毒发病机制。未感染的沙鼠作为对照组。每周收集沙鼠的粪便样本进行逆转录巢式聚合酶链反应。采用酶联免疫吸附测定法检测血清抗HEV IgG和丙氨酸氨基转移酶（ALT）。处死后，收集每只动物的肝、脾和肾进行组织病理学检查。
感染HEV的沙鼠出现乏力，肝、脾和肾出现组织病理学变化。接种后第7天采集的粪便样本中检测到HEV RNA，可检测水平持续至接种后第42天。有趣的是，与未感染的对照组相比，感染HEV的动物中ALT水平仅适度升高。
Z:ZCLA蒙古沙鼠易感人HEV。
