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鱼鳔多糖诱导HCT-116结肠癌细胞凋亡

Induction of apoptosis in HCT-116 colon cancer cells by polysaccharide of swim bladder.

作者信息

Suo Huayi, Song Jia-LE, Zhou Yalin, Liu Zhenhu, Yi Ruokun, Zhu Kai, Xie Jie, Zhao Xin

机构信息

College of Food Science, Southwest University, Chongqing 400715, P.R. China.

Department of Nutrition and Food Hygiene, School of Public Health, Guilin Medical University, Guilin, Guangxi 541004, P.R. China ; Department of Food Science and Nutrition, Pusan National University, Busan 609735, Republic of Korea.

出版信息

Oncol Lett. 2015 Feb;9(2):972-978. doi: 10.3892/ol.2014.2756. Epub 2014 Dec 2.

DOI:10.3892/ol.2014.2756
PMID:25624917
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4301533/
Abstract

swim bladder is a traditional food and medicine widely used in China. The anticancer effects of polysaccharide of swim bladder (PLCSB) in HCT-116 human colon cancer cells was investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. At concentrations ranging between 0 and 800 μg/ml PLCSB, cancer cell viability was decreased by PLCSB in a concentration-dependent manner. In particular, 400 μg/ml PLCSB significantly (P<0.05) induced apoptosis, which was demonstrated by 4,6-diamidino-2-phenylindole staining and flow cytometry analysis. To elucidate the mechanisms underlying the anticancer effect of PLCSB in HCT-116 cancer cells, the expression of apoptosis and metastasis-associated genes was analyzed by reverse transcription-polymerase chain reaction and western blot analysis. A total of 400 μg/ml PLCSB significantly induced apoptosis in HCT-116 cells (P<0.05) via the upregulation Bax, p53, p21, apoptotic protease activating factor 1, caspase-3, -8, and -9, as well as Fas and the downregulation of B-cell lymphoma 2 (Bcl-2), Bcl-extra large and Fas ligand (L). The results of this study demonstrated that PLCSB exhibits an anticancer effect on HCT-116 colon cancer cells, .

摘要

鱼鳔是中国广泛使用的一种传统食品和药物。通过3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐法研究了鱼鳔多糖（PLCSB）对HCT-116人结肠癌细胞的抗癌作用。在0至800μg/ml的PLCSB浓度范围内，癌细胞活力随PLCSB浓度的增加而降低。特别是，400μg/ml的PLCSB显著（P<0.05）诱导细胞凋亡，这通过4,6-二脒基-2-苯基吲哚染色和流式细胞术分析得到证实。为了阐明PLCSB对HCT-116癌细胞抗癌作用的潜在机制，通过逆转录-聚合酶链反应和蛋白质印迹分析对凋亡和转移相关基因的表达进行了分析。总共400μg/ml的PLCSB通过上调Bax、p53、p21、凋亡蛋白酶激活因子1、半胱天冬酶-3、-8和-9以及Fas，并下调B细胞淋巴瘤2（Bcl-2）、Bcl-extra large和Fas配体（L），显著诱导HCT-116细胞凋亡（P<0.05）。本研究结果表明，PLCSB对HCT-116结肠癌细胞具有抗癌作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2e8/4301533/6c552114d309/OL-09-02-0972-g06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2e8/4301533/465afdc6e559/OL-09-02-0972-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2e8/4301533/70311f13d3d8/OL-09-02-0972-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2e8/4301533/21cd92a6b63a/OL-09-02-0972-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2e8/4301533/db20e10709a1/OL-09-02-0972-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2e8/4301533/71c9a0e78641/OL-09-02-0972-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2e8/4301533/84bbf7d91572/OL-09-02-0972-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2e8/4301533/6c552114d309/OL-09-02-0972-g06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2e8/4301533/465afdc6e559/OL-09-02-0972-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2e8/4301533/70311f13d3d8/OL-09-02-0972-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2e8/4301533/21cd92a6b63a/OL-09-02-0972-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2e8/4301533/db20e10709a1/OL-09-02-0972-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2e8/4301533/71c9a0e78641/OL-09-02-0972-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2e8/4301533/84bbf7d91572/OL-09-02-0972-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2e8/4301533/6c552114d309/OL-09-02-0972-g06.jpg

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