Chen Shaocheng, Zhu Kai, Wang Rui, Zhao Xin
Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, P.R. China.
Exp Ther Med. 2014 Jul;8(1):316-322. doi: 10.3892/etm.2014.1712. Epub 2014 May 14.
In the present study the preventive effect of polysaccharides from the large yellow croaker swim bladder (PLYCSB) on HCl/ethanol-induced gastric injury in ICR mice was investigated. A high dose of PLYCSB (50 mg/kg) was found to reduce the levels of the serum proinflammatory cytokines interleukin (IL)-1β, IL-6, IL-8, as well as increase the levels of IL-4 compared with those in mice treated with a low dose of PLYCSB (25 mg/kg) and control mice. The somatostatin and vasoactive intestinal peptide serum levels in PLYCSB-treated mice were higher compared with those in control mice, whilst motilin and substance P serum levels were lower compared with those in control mice. The extent of the gastric injury in the mice treated with PLYCSB was lower compared with that in the control mice; however, the results obtained for mice treated with a high dose of PLYCSB were similar to those for omeprazole-treated mice. In addition, the superoxide dismutase and glutathione peroxidase activities of PLYCSB-treated mice were higher compared with those of the control mice, and similar to those observed in normal and omeprazole-treated mice. Furthermore, PLYCSB-treated mice showed levels of nitric oxide and malondialdehyde that were similar to those in the normal group. Using PCR and western blot analysis, it was demonstrated that PLYCSB significantly inhibited inflammation in the tissues of the HCl/ethanol induced gastric injury mice by downregulating the expression of inducible nitric oxide synthase, cyclooxygenase-2, tumor necrosis factor-α and IL-1β. These results suggest that PLYCSB has an inhibitory effect against gastric injury that is comparable to that of the gastric injury drug omeprazole. Therefore, PLYCSB has the potential to be used as a natural therapeutic drug.
在本研究中，对大黄鱼鱼鳔多糖（PLYCSB）对ICR小鼠盐酸/乙醇诱导的胃损伤的预防作用进行了研究。与低剂量PLYCSB（25mg/kg）处理的小鼠和对照小鼠相比，高剂量PLYCSB（50mg/kg）可降低血清促炎细胞因子白细胞介素（IL）-1β、IL-6、IL-8的水平，并提高IL-4的水平。PLYCSB处理的小鼠的生长抑素和血管活性肠肽血清水平高于对照小鼠，而胃动素和P物质血清水平低于对照小鼠。PLYCSB处理的小鼠的胃损伤程度低于对照小鼠；然而，高剂量PLYCSB处理的小鼠所获得的结果与奥美拉唑处理的小鼠相似。此外，PLYCSB处理的小鼠的超氧化物歧化酶和谷胱甘肽过氧化物酶活性高于对照小鼠，与正常和奥美拉唑处理的小鼠中观察到的活性相似。此外，PLYCSB处理的小鼠的一氧化氮和丙二醛水平与正常组相似。使用PCR和蛋白质印迹分析表明，PLYCSB通过下调诱导型一氧化氮合酶、环氧化酶-2、肿瘤坏死因子-α和IL-1β的表达，显著抑制盐酸/乙醇诱导的胃损伤小鼠组织中的炎症。这些结果表明，PLYCSB对胃损伤具有抑制作用，与胃损伤药物奥美拉唑相当。因此，PLYCSB有潜力用作天然治疗药物。
