Chen Lian-Hong, Song Jia-Le, Qian Yu, Zhao Xin, Suo Hua-Yi, Li Jian
College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China.
Int J Mol Sci. 2014 Jan 9;15(1):817-29. doi: 10.3390/ijms15010817.
The preventive effect of polysaccharide of Larimichthys crocea swimming bladder (PLCSB) and the increase of this effect by use of resistant starch (RS3) as the carrier for PLCSB on azoxymethane (AOM) and dextran sulfate sodium (DSS)-inducing colon carcinogenesis in C57BL/6 mice has been studied. RS3 microspheres carrying PLCSB (RS3 + PLCSB) were produced and evaluated as a potentially improved colon carcinogenesis therapy for this study. The body weight, colon length, and colon weight of mice were determined, and colonic tissues were histologically observed. The serum levels of proinflammatory cytokines and the inflammation and apoptosis-related genes in colonic tissue were also tested. The PLCSB or RS3 + PLCSB significantly suppressed AOM and DSS-induced body weight loss, colon length shortening and decreased the colon weight to length ratio. PLCSB or RS3 + PLCSB reduced the levels of the serum pro-inflammatory cytokines IL-6, IL-12, TNF-α, and IFN-γ to a greater extent compared with the control mice, and the levels of RS3 + PLCSB were more close to the normal mice than PLCSB treated mice. Histopathological examination of sections of colon tissues showed that the RS3 + PLCSB group recovered well from colon carcinogenesis; however, the tissue sections of the stachyose + starch could reduce the necrosis degree. PLCSB significantly induced apoptosis in tissues of mice (p < 0.05) by up-regulating Bax, caspase-3, and caspase-9, and down-regulating Bcl-2. The expression of genes associated with inflammation-related NF-κB, iNOS, and COX-2 genes, was significantly down-regulated, and IκB-α was up-regulated (p < 0.05). These results suggest that PLCSB is a potent preventive against in vivo colon carcinogenesis and that PLCSB with an RS3 carrier could increase the preventative effect in mice.
研究了大黄鱼鱼鳔多糖（PLCSB）的预防作用以及使用抗性淀粉（RS3）作为PLCSB的载体对其预防作用的增强效果，该预防作用针对的是在C57BL/6小鼠中由氧化偶氮甲烷（AOM）和葡聚糖硫酸钠（DSS）诱导的结肠癌发生。制备了携带PLCSB的RS3微球（RS3 + PLCSB）并将其评估为该研究中一种潜在的改良结肠癌治疗方法。测定了小鼠的体重、结肠长度和结肠重量，并对结肠组织进行了组织学观察。还检测了血清促炎细胞因子水平以及结肠组织中与炎症和凋亡相关的基因。PLCSB或RS3 + PLCSB显著抑制了AOM和DSS诱导的体重减轻、结肠长度缩短，并降低了结肠重量与长度的比值。与对照小鼠相比，PLCSB或RS3 + PLCSB更大程度地降低了血清促炎细胞因子IL-6、IL-12、TNF-α和IFN-γ的水平，并且RS3 + PLCSB组的水平比PLCSB处理组的小鼠更接近正常小鼠。结肠组织切片的组织病理学检查表明，RS3 + PLCSB组从结肠癌发生中恢复良好；然而，水苏糖+淀粉的组织切片可降低坏死程度。PLCSB通过上调Bax、caspase-3和caspase-9并下调Bcl-2，显著诱导小鼠组织中的细胞凋亡（p < 0.05）。与炎症相关的NF-κB、iNOS和COX-2基因的表达显著下调，而IκB-α上调（p < 0.05）。这些结果表明，PLCSB对体内结肠癌发生具有强大的预防作用，并且带有RS3载体的PLCSB可增强对小鼠的预防效果。
