Yoon Sang Sun, Kim Eun-Kyoung, Lee Won-Jae
Department of Microbiology and Immunology, Institute for Immunology and Immunological Diseases, Yonsei University College of Medicine, Seoul 120-752, South Korea.
School of Biological Science and Institute of Molecular Biology and Genetics, Seoul National University, Seoul 151-742, South Korea; Seoul National University, National Creative Research Initiative Center for Symbiosystem, Seoul 151-742, South Korea.
Curr Opin Microbiol. 2015 Apr;24:38-46. doi: 10.1016/j.mib.2015.01.007. Epub 2015 Jan 24.
Accumulating data sets of gut microbiome by next-generation sequencing allow us to gain a comprehensive view of the functional diversity of the gut-associated metagenome. However, many microbiome functions are unknown and/or have only been predicted, and may not necessarily reflect the in vivo function within a gut niche. Functional genomic and metagenomic approaches have been successfully applied to broaden the understanding of invertebrate and vertebrate gut microbiome involved in diverse functions, including colonization ability, nutritional processing, antibiotic resistance, microbial physiology and metabolism, and the modulation of the host physiology. In this review, we discuss the recent knowledge obtained from the study of functional genomics and metagenomics of the animal intestine and its potential values for understanding gut microbiota-animal mutualism.
通过下一代测序积累的肠道微生物组数据集，使我们能够全面了解肠道相关宏基因组的功能多样性。然而，许多微生物组功能尚不清楚和/或仅为预测所得，不一定能反映肠道生态位内的体内功能。功能基因组学和宏基因组学方法已成功应用于拓宽对参与多种功能的无脊椎动物和脊椎动物肠道微生物组的理解，这些功能包括定殖能力、营养加工、抗生素抗性、微生物生理学和代谢以及宿主生理学的调节。在本综述中，我们讨论了从动物肠道功能基因组学和宏基因组学研究中获得的最新知识及其在理解肠道微生物群与动物共生关系方面的潜在价值。
