CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa/Porto, Rua Dr. António Bernardino Almeida, 4200-072 Porto, Portugal.
CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa/Porto, Rua Dr. António Bernardino Almeida, 4200-072 Porto, Portugal; Centro de Química Medicinal da Universidade do Porto (CEQUIMED-UP), Rua de Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal; CESPU, Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde, Rua Central de Gandra 1317, 4585-116 Gandra PRD, Portugal.
J Hazard Mater. 2015 Apr 28;287:93-101. doi: 10.1016/j.jhazmat.2015.01.020. Epub 2015 Jan 8.
Fluoxetine (FLX) is a chiral fluorinated pharmaceutical mainly indicated for treatment of depression and is one of the most distributed drugs. There is a clear evidence of environmental contamination with this drug. Aerobic granular sludge sequencing batch reactors constitute a promising technology for wastewater treatment; however the removal of carbon and nutrients can be affected by micropollutants. In this study, the fate and effect of FLX on reactor performance and on microbial population were investigated. FLX adsorption/desorption to the aerobic granules was observed. FLX shock loads (≤4μM) did not show a significant effect on the COD removal. Ammonium removal efficiency decreased in the beginning of first shock load, but after 20 days, ammonia oxidizing bacteria became adapted. The nitrite concentration in the effluent was practically null indicating that nitrite oxidizing bacteria was not inhibited, whereas, nitrate was accumulated in the effluent, indicating that denitrification was affected. Phosphate removal was affected at the beginning showing a gradual adaptation, and the effluent concentration was <0.04mM after 70 days. A shift in microbial community occurred probably due to FLX exposure, which induced adaptation/restructuration of the microbial population. This contributed to the robustness of the reactor, which was able to adapt to the FLX load.
氟西汀（FLX）是一种手性氟化药物，主要用于治疗抑郁症，是分布最广的药物之一。有明确的证据表明这种药物会造成环境污染。好氧颗粒污泥序批式反应器是一种很有前途的废水处理技术；然而，碳和营养物质的去除可能会受到微量污染物的影响。本研究考察了 FLX 在反应器性能和微生物种群中的去向和作用。观察到 FLX 对好氧颗粒的吸附/解吸。FLX 冲击负荷（≤4μM）对 COD 去除率没有显著影响。在第一次冲击负荷开始时，氨氮去除效率降低，但 20 天后，氨氧化菌适应了。出水中的亚硝酸盐浓度几乎为零，表明亚硝酸盐氧化菌未受抑制，而硝酸盐在出水中积累，表明反硝化受到影响。在开始时，磷的去除受到影响，随后逐渐适应，70 天后出水中的浓度<0.04mM。由于 FLX 的暴露，微生物群落发生了变化，这可能导致了微生物种群的适应/重构。这有助于提高反应器的鲁棒性，使其能够适应 FLX 负荷。
