Environmental Engineering Laboratory, Department of Civil Engineering, University of Patras, 26504 Patras, Greece.
Section of Animal Biology, Department of Biology, Faculty of Sciences, University of Patras, 26504 Patras, Greece.
Ecotoxicol Environ Saf. 2015 Apr;114:109-16. doi: 10.1016/j.ecoenv.2015.01.016. Epub 2015 Jan 24.
The aim of this work was to investigate the effect of zinc oxide nanoparticles (ZnO NPs) on freshwater and marine microalgae cultivated in different media. Freshwater species Chlorococcum sp. and Scenedesmus rubescens were cultivated in modified Blue-Green medium (BG-11) and Bold's Basal Medium (BBM), and marine species Dunaliella tertiolecta, and Tetraselmis suesica, cultured in salt modified BG-11 and f/2 medium. The microalgae species were exposed for 96 h with a daily reading of algal growth rate, to different ZnO NPs concentrations (0.081-810 mg/L). Significant differences were observed on microalgae growth rates, with the marine being more sensitive than the freshwater species, as revealed by their half inhibitory concentration values (IC50). The IC50 values in freshwater species were affected by the culture medium. The lowest IC50 values (<2.57 mg/L) were observed in the marine species. S. rubescens showed the less toxic effect in cultures with modified BG-11, compared to BBM cultures, with IC50 values >810 mg/L and 14.27 mg/L after 96 h exposure time, respectively. ZnO nanoparticles appeared to have toxic effects in all species tested, depended on the species type, the exposure time, the NPs concentration, and mainly the culture medium.
本研究旨在探讨氧化锌纳米粒子（ZnO NPs）对不同培养基中培养的淡水和海洋微藻的影响。淡水物种绿球藻（Chlorococcum sp.）和栅藻（Scenedesmus rubescens）在改良蓝绿藻培养基（BG-11）和 Bold 基础培养基（BBM）中培养，海洋物种杜氏盐藻（Dunaliella tertiolecta）和三角褐指藻（Tetraselmis suesica）在改良盐 BG-11 和 f/2 培养基中培养。将微藻物种暴露于不同 ZnO NPs 浓度（0.081-810 mg/L）下 96 小时，每天读取藻类生长率。结果表明，微藻的生长率存在显著差异，海洋物种比淡水物种更敏感，其半抑制浓度（IC50）值也证实了这一点。在淡水物种中，IC50 值受培养基的影响。在海洋物种中观察到的最低 IC50 值（<2.57 mg/L）。与 BBM 培养物相比，S. rubescens 在改良 BG-11 培养物中表现出较低的毒性效应，暴露 96 小时后的 IC50 值分别为>810 mg/L 和 14.27 mg/L。ZnO 纳米粒子似乎对所有测试的物种都有毒性作用，这取决于物种类型、暴露时间、NPs 浓度以及主要的培养基。
