Geddes Barney A, Ryu Min-Hyung, Mus Florence, Garcia Costas Amaya, Peters John W, Voigt Christopher A, Poole Philip
Department of Plant Sciences, Oxford University, Oxford OX1 3RB, United Kingdom.
Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Curr Opin Biotechnol. 2015 Apr;32:216-222. doi: 10.1016/j.copbio.2015.01.004. Epub 2015 Jan 24.
Engineering cereal crops that are self-supported by nitrogen fixation has been a dream since the 1970s when nitrogenase was transferred from Klebsiella pneumoniae to Escherichia coli. A renewed interest in this area has generated several new approaches with the common aim of transferring nitrogen fixation to cereal crops. Advances in synthetic biology have afforded the tools to rationally engineer microorganisms with traits of interest. Nitrogenase biosynthesis has been a recent target for the application of new synthetic engineering tools. Early successes in this area suggest that the transfer of nitrogenase and other supporting traits to microorganisms that already closely associate with cereal crops is a logical approach to deliver nitrogen to cereal crops.
自20世纪70年代将固氮酶从肺炎克雷伯菌转移到大肠杆菌以来，培育能够通过固氮实现自我供养的谷类作物一直是一个梦想。对这一领域重新燃起的兴趣催生了几种新方法，其共同目标是将固氮能力转移到谷类作物上。合成生物学的进展提供了工具，能够合理地改造具有所需性状的微生物。固氮酶生物合成一直是新型合成工程工具应用的一个近期目标。该领域早期取得的成功表明，将固氮酶和其他支持性状转移到已经与谷类作物紧密关联的微生物上，是向谷类作物输送氮的合理途径。
