Lima Lionete N, Oliveira Gladson C, Rojas Mayerlenis J, Castro Heizir F, Da Rós Patrícia C M, Mendes Adriano A, Giordano Raquel L C, Tardioli Paulo W
Graduate Program in Chemical Engineering, Federal University of São Carlos, Rodovia Washington Luiz, km 235, São Carlos, SP, 13565-905, Brazil.
J Ind Microbiol Biotechnol. 2015 Apr;42(4):523-35. doi: 10.1007/s10295-015-1586-9. Epub 2015 Jan 28.
This work describes the preparation of biocatalysts for ethanolysis of soybean and babassu oils in solvent-free systems. Polystyrene, Amberlite (XAD-7HP), and octyl-silica were tested as supports for the immobilization of Pseudomonas fluorescens lipase (PFL). The use of octyl-silica resulted in a biocatalyst with high values of hydrolytic activity (650.0 ± 15.5 IU/g), immobilization yield (91.3 ± 0.3 %), and recovered activity (82.1 ± 1.5 %). PFL immobilized on octyl-silica was around 12-fold more stable than soluble PFL, at 45 °C and pH 8.0, in the presence of ethanol at 36 % (v/v). The biocatalyst provided high vegetable oil transesterification yields of around 97.5 % after 24 h of reaction using babassu oil and around 80 % after 48 h of reaction using soybean oil. The PFL-octyl-silica biocatalyst retained around 90 % of its initial activity after five cycles of transesterification of soybean oil. Octyl-silica is a promising support that can be used to immobilize PFL for subsequent application in biodiesel synthesis.
这项工作描述了在无溶剂体系中制备用于大豆油和巴巴苏油乙醇解的生物催化剂的过程。测试了聚苯乙烯、Amberlite（XAD - 7HP）和辛基硅胶作为固定化荧光假单胞菌脂肪酶（PFL）的载体。使用辛基硅胶得到了一种生物催化剂，其具有高水解活性值（650.0±15.5 IU/g）、固定化产率（91.3±0.3%）和回收活性（82.1±1.5%）。在45°C、pH 8.0且存在36%（v/v）乙醇的条件下，固定在辛基硅胶上的PFL比可溶性PFL稳定约12倍。使用巴巴苏油反应24小时后，该生物催化剂的植物油酯交换产率较高，约为97.5%；使用大豆油反应48小时后，产率约为80%。大豆油酯交换五个循环后，PFL - 辛基硅胶生物催化剂保留了约90%的初始活性。辛基硅胶是一种有前景的载体，可用于固定PFL，以便后续应用于生物柴油合成。
