Oliveira Karoliny Almeida, de Souza Fabrício Ribeiro, de Oliveira Cristina Rodrigues, da Silveira Lucimeire Antonelli, Coltro Wendell Karlos Tomazelli
Instituto de Química, Universidade Federal de Goiás, Campus Samambaia, 131, Goiânia, GO, 74001-970, Brazil.
Methods Mol Biol. 2015;1256:85-98. doi: 10.1007/978-1-4939-2172-0_6.
This chapter describes the development of microfluidic toner-based analytical devices (μTADs) to perform clinical diagnostics using a scanner or cell-phone camera. μTADs have been produced in a platform composed of polyester and toner by the direct-printing technology (DPT) in a matter of minutes. This technology offers simplicity and versatility, and it does not require any sophisticated instrumentation. Toner-based devices integrate the current generation of disposable analytical devices along paper-based chips. The cost of one μTAD has been estimated to be lower than $0.10. In addition, these platforms are lightweight and portable thus enabling their use for point-of-care applications. In the last 5 years, great efforts have been dedicated to spread out the use of μTADs in bioassays. The current chapter reports the fabrication of printed microplates and integrated microfluidic toner-based devices for dengue diagnostics and rapid colorimetric assays with clinically relevant analytes including cholesterol, triglycerides, total proteins, and glucose. The use of μTADs associated with cell-phone camera may contribute to the health care, in special, to people housed in developing regions or with limited access to clinics and hospitals.
本章介绍了基于微流体墨粉的分析设备（μTADs）的发展，该设备可使用扫描仪或手机摄像头进行临床诊断。通过直接打印技术（DPT），在几分钟内就能在由聚酯和墨粉组成的平台上制造出μTADs。这项技术操作简单且用途广泛，不需要任何复杂的仪器。基于墨粉的设备将当前一代一次性分析设备与纸质芯片整合在一起。据估计，一个μTAD的成本低于0.10美元。此外，这些平台轻便易携带，因此可用于即时检测应用。在过去5年里，人们付出了巨大努力来推广μTADs在生物检测中的应用。本章报告了用于登革热诊断的印刷微孔板和集成微流体墨粉基设备的制造，以及针对包括胆固醇、甘油三酯、总蛋白和葡萄糖在内的具有临床相关性的分析物的快速比色测定。将μTADs与手机摄像头结合使用可能有助于医疗保健，特别是对生活在发展中地区或就医机会有限的人群。
