Delaney Jacqui L, Hogan Conor F
Department of Chemistry, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC, 3086, Australia.
Methods Mol Biol. 2015;1256:277-89. doi: 10.1007/978-1-4939-2172-0_19.
The development of simple, inexpensive paper-based sensors for medical diagnostics and other applications is now an important emerging area in the field of biosensors; however, the electronic instrument or reader used to interrogate such sensors adds significantly to the cost of the analysis. In this chapter we describe the design and construction of novel, low-cost disposable electrochemiluminescent (ECL) sensors based on screen printed carbon electrodes and paper-based microfluidics. Moreover, a method to interrogate these sensors using only a mobile phone is articulated. This is realized by exploiting the audio output of the device to achieve electrochemical control, while using the camera to detect the resulting light emitted during the ECL reaction. The combination of cell phone technology with low-cost paper microfluidic sensors dramatically reduces the cost of sensing and has the potential to enhance health-care outcomes by exploiting the functionality, connectivity, and close to worldwide penetration of mobile phone technology.
开发用于医学诊断及其他应用的简单、廉价的纸质传感器，如今已成为生物传感器领域一个重要的新兴领域；然而，用于检测此类传感器的电子仪器或读取器会显著增加分析成本。在本章中，我们描述了基于丝网印刷碳电极和纸质微流控技术的新型低成本一次性电化学发光（ECL）传感器的设计与构建。此外，还阐述了一种仅使用手机来检测这些传感器的方法。这是通过利用设备的音频输出实现电化学控制，同时使用摄像头检测ECL反应过程中产生的光来实现的。手机技术与低成本纸质微流控传感器的结合极大地降低了传感成本，并且通过利用手机技术的功能、连接性以及近乎全球普及的特性，有潜力改善医疗保健效果。
