Tabarsa Mehdi, Park Geun-Mook, Shin Il-Shik, Lee EunJung, Kim Jin-Kyung, You SangGuan
Department of Marine Food Science and Technology, Gangneung-Wonju National University, 120 Gangneungdaehangno, Gangneung, Gangwon, 210-702, South Korea.
Mar Biotechnol (NY). 2015 Jun;17(3):266-76. doi: 10.1007/s10126-015-9615-2. Epub 2015 Jan 28.
The effects of sulfate and protein contents as well as molecular weights of the sulfated glycoproteins (NF2) from Codium fragile on the immunomodulation were systematically investigated. The obtained NF2 derivatives displayed various amounts of proteins (2.3-8.7 %) and sulfates (4.3-8.1 %) as well as different molecular weights (47.3-128.0 × 10(3) g/mol). NF2 was not able to stimulate RAW264.7 cells to release NO without its protein moiety, which was essential to activate NF-κB pathway through the degradation and phosphorylation of IκB-α and the subsequent translocation of p65/p50 complex in the cell nucleus. In addition, the proteins in NF2 were required to trigger MAPK pathway for the phosphorylation of ERK1/2, p38, and JNK1/2 as well as the nuclear translocation of c-JUN and c-FOS. However, the protein moiety itself could not activate RAW264.7 cells, thus the complex formation of the polysaccharide and protein moieties in NF2 was pivotal to stimulate macrophage cells.
系统研究了来自脆弱刚毛藻的硫酸化糖蛋白（NF2）的硫酸盐和蛋白质含量以及分子量对免疫调节的影响。所获得的NF2衍生物表现出不同量的蛋白质（2.3 - 8.7%）和硫酸盐（4.3 - 8.1%）以及不同的分子量（47.3 - 128.0×10³ g/mol）。NF2在没有其蛋白质部分时无法刺激RAW264.7细胞释放NO，该蛋白质部分对于通过IκB-α的降解和磷酸化以及随后p65/p50复合物在细胞核中的转位来激活NF-κB途径至关重要。此外，NF2中的蛋白质是触发MAPK途径使ERK1/2、p38和JNK1/2磷酸化以及c-JUN和c-FOS核转位所必需的。然而，蛋白质部分本身不能激活RAW264.7细胞，因此NF2中多糖和蛋白质部分的复合物形成对于刺激巨噬细胞至关重要。
