Department of Seafood Processing, Faculty of Marine Sciences, Tarbiat Modares University, P.O. Box 46414-356, Noor, Iran.
Department of Marine Food Science and Technology, Gangneung-Wonju National University, Gangneung, Gangwon 25457, Republic of Korea.
J Food Drug Anal. 2018 Apr;26(2):599-608. doi: 10.1016/j.jfda.2017.07.016. Epub 2017 Aug 18.
Water-soluble sulfated polysaccharides extracted from Ulva intestinalis and fractionated using DEAE Sepharose fast flow column to identify their molecular properties and macrophage cells stimulating activities. Crude and fractions (F and F) were formed of neutral sugars (58.7-74.7%), sulfates (6.2-24.5%), uronic acids (4.9-5.9%) and proteins (3.2-10.4%). Different levels of sugar constituents including rhamnose (30.1-39.1%), glucose (39.0-48.4%), galactose (0.0-15.8%), xylose (8.5-11.3) and arabinose (0.0-5.1%). The molecular weight (M) of crude and fractionated polysaccharides ranged from 87.1 × 10 to 194.1 × 10 (g/mol). Crude polysaccharides were not toxic to RAW264.7 cells and fractions induced cell proliferation. Fraction F stimulated RAW264.7 cells to release considerable amounts of nitric oxide, IL-1β, TNF-α, IL-6, IL-10 and IL-12 cytokines. The main backbone of the most immunostimulating polysaccharide (F) was consisted of mixed linkages of (1 → 2)-linked rhamnose and (1 → 2)-linked glucose residues.
从肠浒苔中提取的水溶性硫酸化多糖，并用 DEAE Sepharose fast flow 柱进行分级，以鉴定其分子特性和巨噬细胞刺激活性。粗品和级分（F 和 F）由中性糖（58.7-74.7%）、硫酸盐（6.2-24.5%）、糖醛酸（4.9-5.9%）和蛋白质（3.2-10.4%）组成。不同水平的糖成分，包括鼠李糖（30.1-39.1%）、葡萄糖（39.0-48.4%）、半乳糖（0.0-15.8%）、木糖（8.5-11.3%）和阿拉伯糖（0.0-5.1%）。粗多糖和分级多糖的分子量（M）范围为 87.1×10 至 194.1×10（g/mol）。粗多糖对 RAW264.7 细胞无毒，级分可诱导细胞增殖。级分 F 刺激 RAW264.7 细胞释放大量的一氧化氮、IL-1β、TNF-α、IL-6、IL-10 和 IL-12 细胞因子。最具免疫刺激作用的多糖（F）的主要骨架由混合键连接的（1→2）-连接的鼠李糖和（1→2）-连接的葡萄糖残基组成。
