Ishnava Kalpesh B, Shah Pankit P
Assistant Professor, Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied Sciences (ARIBAS), New VallabhVidyanagar, Gujarat, India.
J Dent (Tehran). 2014 Sep;11(5):576-86. Epub 2014 Sep 30.
This study aimed to assess the anticariogenic and hemolytic activity of crude plant seed protein extracts against tooth decaying bacteria.
The proteins from seeds of 12 different plants were extracted and used for antimicrobial assay against six different organisms. The extraction was carried out in 10mM of sodium phosphate buffer (pH 7.0). Protein concentrations were determined as described by Bradford method. Anticariogenic activity was studied by agar well diffusion method and Minimum Inhibitory Concentration (MIC) was evaluated by the two-fold serial broth dilution method. Hemolytic activity, treatment of proteinase K and Kinetic study in Mimusops elengi crude seed protein extract.
The anticariogenic assay demonstrated the activity of Mimusops elengi against Staphylococcus aureus and Streptococcus pyogenes. A minor activity of Glycine wightii against Streptococcus mutans was also found. The protein content of Mimusops elengi seed protein extract was 5.84mg/ml. The MIC values for Staphylococcus aureus and Streptococcus pyogenes against Mimusops elengi seed protein extract were 364.36μg/ml and 182.19μg/ml, respectively. Kinetic study further elucidated the mode of inhibition in the presence of the Mimusops elengi plant seed protein with respect to time. The concentration of crude extract which gave 50% hemolysis compared to Triton X-100 treatment (HC50) value was 1.58 mg/ml; which is more than five times larger than that of the MIC. Treatment with proteinase K of the Mimusops elengi seed protein resulted in absence of the inhibition zone; which clearly indicates that the activity was only due to protein.
Our results showed the prominence of Mimusops elengi plant seed protein extract as an effective herbal medication against tooth decaying bacteria.
本研究旨在评估植物种子粗蛋白提取物对致龋菌的防龋和溶血活性。
提取12种不同植物种子中的蛋白质，并用于针对6种不同微生物的抗菌试验。提取在10mM磷酸钠缓冲液（pH 7.0）中进行。按照Bradford法测定蛋白质浓度。采用琼脂孔扩散法研究防龋活性，通过两倍系列肉汤稀释法评估最低抑菌浓度（MIC）。对人心果粗种子蛋白提取物进行溶血活性、蛋白酶K处理及动力学研究。
防龋试验表明人心果对金黄色葡萄球菌和化脓性链球菌具有活性。还发现白花灰叶豆对变形链球菌有轻微活性。人心果种子蛋白提取物的蛋白质含量为5.84mg/ml。金黄色葡萄球菌和化脓性链球菌对人心果种子蛋白提取物的MIC值分别为364.36μg/ml和182.19μg/ml。动力学研究进一步阐明了在存在人心果植物种子蛋白的情况下抑制作用随时间的模式。与Triton X - 100处理相比，产生50%溶血的粗提物浓度（HC50）值为1.58mg/ml；该值比MIC值大五倍多。用蛋白酶K处理人心果种子蛋白后，抑菌圈消失；这清楚地表明活性仅归因于蛋白质。
我们的结果表明人心果植物种子蛋白提取物作为一种有效的抗致龋菌草药药物具有显著作用。
