AbdElgawad Hamada, Magdy Korany Shereen, Hagagy Nashwa, Yaghoubi Khanghahi Mohammad, Reyad Ahmed Mohamed, Crecchio Carmine, Zakri Adel M, Alsherif Emad A, Bakkar Marwa Reda
Integrated Molecular Plant Physiology Research (IMPRES), Department of Biology, University of Antwerp, Antwerp, Belgium.
Botany and Microbiology Department, Faculty of Science, Beni-Suef University, Beni Suef, 62521 Egypt.
3 Biotech. 2023 Dec;13(12):412. doi: 10.1007/s13205-023-03836-0. Epub 2023 Nov 22.
The present research aimed to understand the influence of plant growth-promoting bacteria (PGPB) on various biochemical, nutritional, and pharmaceutical characteristics of plants grown under elevated carbon dioxide (eCO). To achieve this objective, a pot experiment was carried out, consisting of two treatments, namely: (i) biofertilization (Bf) by a PGPB strain ( sp.) and (ii) two different air CO levels, including ambient CO (aCO) and eCO concentrations (410 and 710 μmol CO mol, respectively). The improvement in the photosynthesis rate of eCO and Bf-treated plants can explain the increase in the production of carbohydrate. This is evidenced by a substantial rise, reaching up to + 75% and 25% in the total sugar and starch content in plants subjected to eCO treatment, respectively. Additionally, eCO-treated plants exhibited a remarkable 102% increase in soluble sugar synthesis, while plants subjected to Bf treatment showed a notable increase of 66%. Such modifications could be the main factor affecting plants carbon and nitrogen metabolism. Although the level of certain amino acids (such as glycine, tyrosine, and phenylalanine) in plants exhibited significant increases in response to eCO and Bf, the levels of other amino acids demonstrated enhancements in plants grown under eCO (e.g., histidine) or under treatments containing Bf (e.g., alanine and ornithine). Improvements in primary metabolites led to more benefits in plants treated with Bf and CO by boosting secondary metabolites accumulation, including phenolics (+ 27-100%), flavonoids (+ 30-92%), and essential oils (up to + 296%), as well as improved antioxidant capacity (FRAP). This remarkable effectiveness was evident in the significant increase in the biomass production, highlighting the synergistic impact of the treatments. Therefore, the interaction of Bf and eCO not only induced plant biomass accumulation but also improved the nutritional and pharmaceutical value of plants.
本研究旨在了解促植物生长细菌（PGPB）对在高二氧化碳（eCO）环境下生长的植物的各种生化、营养和药用特性的影响。为实现这一目标，进行了一项盆栽试验，包括两种处理，即：（i）用一株PGPB菌株（sp.）进行生物施肥（Bf），以及（ii）两种不同的大气CO₂水平，包括环境CO₂（aCO₂）和eCO₂浓度（分别为410和710 μmol CO₂/mol）。eCO₂处理和Bf处理的植物光合作用速率的提高可以解释碳水化合物产量的增加。这一点在eCO₂处理的植物中总糖和淀粉含量分别大幅上升高达+75%和25%中得到证明。此外，eCO₂处理的植物可溶性糖合成显著增加了102%，而Bf处理的植物则显著增加了66%。这种变化可能是影响植物碳和氮代谢的主要因素。尽管植物中某些氨基酸（如甘氨酸、酪氨酸和苯丙氨酸）的水平在eCO₂和Bf处理下显著增加，但其他氨基酸的水平在eCO₂环境下生长的植物（如组氨酸）或含有Bf的处理（如丙氨酸和鸟氨酸）下生长的植物中有所提高。初级代谢产物的改善通过促进次生代谢产物的积累，包括酚类物质（+27 - 100%）、黄酮类化合物（+30 - 92%）和精油（高达+296%），以及提高抗氧化能力（FRAP），在Bf和CO₂处理的植物中带来了更多益处。这种显著的效果在生物量产量的显著增加中很明显，突出了这些处理的协同作用。因此，Bf和eCO₂的相互作用不仅诱导了植物生物量的积累，还提高了植物的营养和药用价值。
