利用微核和其他核异常评估职业性接触多环芳烃的汽车修理工的遗传毒性。
Evaluation of genotoxicity in automobile mechanics occupationally exposed to polycyclic aromatic hydrocarbons using micronuclei and other nuclear abnormalities.
作者信息
Khan Mohammed Rafiq, Sudha Sellappa
机构信息
Dept. of Biotechnology, Molecular Diagnosis and Drug Discovery Laboratory, School of Life Sciences, Karpagam University, Coimbatore, Tamilnadu, India.
出版信息
Iran J Cancer Prev. 2012 Spring;5(2):87-92.
BACKGROUND
Occupational and environmental exposures mostly represent mixtures of genotoxic agents, whereas the specificity of biomarker measurements varies widely. Exploration of correlations among biomarkers contributes to the further progress of molecular cancer epidemiology and to the selection of the optimal biomarkers for the investigation of human exposure to carcinogens. The aim of this study was to assess the potential cytogenetic damage associated with occupational exposure to Polycyclic Aromatic Hydrocarbons (PAHs) among automobile mechanics by using Micronuclei (MN) and other Nuclear Abnormalities (NA) as a biomarker.
METHODS
The study population composed of 110 occupationally exposed automobile mechanics and 100 unexposed controls. All the study participants were males. Both the exposed and control individuals were selected from automobile garages located in the urban area of Coimbatore City, South India. Exfoliated buccal cells were collected from 110 automobile mechanics and 100 age and sex matched controls. Further, cells were examined for MN frequency and Nuclear Abnormalities (NA) other than micronuclei, such as binucleates, broken eggs and karyolysis.
RESULTS
Results showed a statistically significant difference between occupationally exposed automobile mechanics and control groups. MN and NA frequencies in automobile mechanics were significantly higher than those in control groups (p < 0.05) and also significantly related to smoking habit (p < 0.05). In addition, a higher degree of NA was observed among the exposed subjects with smoking, drinking, tobacco chewing, which is an indicative of cytogenetic damage in these individuals.
CONCLUSION
MN and other NA reflect genetic changes, events associated with carcinogenesis. Therefore, the results of this study indicate that automobile mechanics exposed to PAHs are under risk of significant cytogenetic damage. Therefore, it is important to provide and offer better awareness of occupational hazards among these workers to promote occupational safety.
背景
职业和环境暴露大多代表基因毒性剂的混合物，而生物标志物测量的特异性差异很大。探索生物标志物之间的相关性有助于分子癌症流行病学的进一步发展，并有助于选择用于调查人类接触致癌物的最佳生物标志物。本研究的目的是通过使用微核（MN）和其他核异常（NA）作为生物标志物，评估汽车修理工职业接触多环芳烃（PAHs）相关的潜在细胞遗传损伤。
方法
研究人群由110名职业暴露的汽车修理工和100名未暴露的对照组成。所有研究参与者均为男性。暴露组和对照组均选自印度南部哥印拜陀市市区的汽车修理店。从110名汽车修理工和100名年龄和性别匹配的对照中收集脱落的颊细胞。此外，检查细胞的MN频率和除微核外的核异常（NA），如双核、碎核和核溶解。
结果
结果显示职业暴露的汽车修理工与对照组之间存在统计学上的显著差异。汽车修理工的MN和NA频率显著高于对照组（p < 0.05），并且也与吸烟习惯显著相关（p < 0.05）。此外，在有吸烟、饮酒、嚼烟习惯的暴露受试者中观察到更高程度的NA，这表明这些个体存在细胞遗传损伤。
结论
MN和其他NA反映了与致癌作用相关的遗传变化和事件。因此，本研究结果表明，接触PAHs的汽车修理工存在显著细胞遗传损伤的风险。因此，提高这些工人对职业危害的认识以促进职业安全非常重要。
Zotero 插件