O'Gorman M R, Oger J J
Department of Pathology, University of British Columbia, Vancouver, Canada.
Cell Immunol. 1989 Feb;118(2):435-47. doi: 10.1016/0008-8749(89)90391-2.
PWM-induced differentiation of human PBMC into immunoglobulin (Ig) secreting cells is a widely used model of in vivo IgG secretion. We examined the relationship between the amount of IgG secreted in PBMC cultures obtained from individuals who consistently secrete either very high (HR) or very low amounts (LR) of IgG and various in vitro immune function assays. The PBMC obtained from LR contained a higher ratio of cells expressing the T-suppressor/inducer to T-helper/inducer phenotype. The autologous mixed lymphocyte response was lower and the amount of in vivo radiation sensitive suppression was higher in the LR than in the HR. LR E- cells secreted less IgG than the HR E- cells when both were mixed with heterologous HR E+ cells. Monocyte depletion reduced IgG secretion in both LR and HR. These results suggest that each of the subsets Ts, Th (Thi, Tsi), and B lymphocytes are involved in the regulation of PWM-induced Ig secretion and that the functions of each of these subsets differ in HR and LR individuals.
PWM诱导人外周血单个核细胞分化为免疫球蛋白（Ig）分泌细胞是一种广泛应用的体内IgG分泌模型。我们研究了从持续分泌非常高（HR）或非常低（LR）量IgG的个体获得的PBMC培养物中分泌的IgG量与各种体外免疫功能测定之间的关系。从LR个体获得的PBMC中，表达T抑制/诱导型与T辅助/诱导型表型的细胞比例更高。LR个体的自体混合淋巴细胞反应较低，体内辐射敏感性抑制量高于HR个体。当LR E-细胞和HR E-细胞与异源HR E+细胞混合时，LR E-细胞分泌的IgG比HR E-细胞少。单核细胞耗竭减少了LR和HR个体中的IgG分泌。这些结果表明，Ts、Th（Thi、Tsi）和B淋巴细胞亚群均参与PWM诱导的Ig分泌调节，且这些亚群在HR和LR个体中的功能有所不同。
