Goldstein R, Perelmutter L, Karsh J
Division of Rheumatology and Clinical Immunology, Ottawa General Hospital, University of Ottawa, Ontario, Canada.
Clin Exp Immunol. 1987 Aug;69(2):358-67.
Staphylococcus aureus Cowan I (SAC) is a potent stimulus of B cell proliferation and differentiation, the latter being T cell-dependent. It has been suggested that immunoglobulin and IgM rheumatoid factor (RF) production in response to SAC involves radiosensitive T helper cells. We studied normal peripheral blood mononuclear cell (PBMC) cultures to assess the roles of radiosensitive T cells and Leu-2 positive suppressor cells in the cellular control of SAC-stimulated IgM and IgM RF responses. Depletion of Leu-2 positive T cells from reconstituted autologous PBMC cultures resulted in an increase in SAC-stimulated IgM production in the majority of individuals, implying the involvement of Leu-2 positive suppressor T cells in this system. Suppression by Leu-2 positive cells is less evident in the SAC-induced IgM RF response, suggesting qualitative differences between IgM and IgM RF SAC-stimulated responses in PBMC cultures from the same normal individuals. Irradiation (1000 rads) of the T cell-enriched subpopulation, either with or without Leu-2 positive cell depletion, resulted in statistically significant decreases in IgM and IgM RF production in response to SAC in reconstituted autologous cultures, providing further evidence of a Leu-2 negative radiosensitive sheep-cell rosetting cell active in in vitro SAC responses. In contrast, PWM-stimulated PBMC cultures showed almost exclusively increases in total IgM and IgM RF production when T cells were irradiated (1000 rads) before culture, consistent with the radiosensitive T suppressor cell involved in the in vitro immunoglobulin responses to PWM. The same five out of nine individuals produced IgM RF, under different culture conditions, in response to PWM and SAC, suggesting that the ability of an individual to produce IgM RF lies intrinsically within the B cell.
金黄色葡萄球菌考恩I型（SAC）是B细胞增殖和分化的有效刺激物，后者依赖于T细胞。有人提出，对SAC产生免疫球蛋白和IgM类风湿因子（RF）涉及放射敏感的T辅助细胞。我们研究了正常外周血单个核细胞（PBMC）培养物，以评估放射敏感T细胞和Leu-2阳性抑制细胞在SAC刺激的IgM和IgM RF反应的细胞控制中的作用。从重建的自体PBMC培养物中去除Leu-2阳性T细胞导致大多数个体中SAC刺激的IgM产生增加，这意味着Leu-2阳性抑制性T细胞参与了该系统。Leu-2阳性细胞在SAC诱导的IgM RF反应中的抑制作用不太明显，这表明来自相同正常个体的PBMC培养物中IgM和IgM RF SAC刺激反应之间存在质的差异。对富含T细胞的亚群进行照射（1000拉德），无论是否去除Leu-2阳性细胞，在重建的自体培养物中，对SAC的IgM和IgM RF产生均有统计学意义的下降，这进一步证明了一种Leu-2阴性的放射敏感绵羊红细胞花环形成细胞在体外SAC反应中具有活性。相反，当在培养前对T细胞进行照射（1000拉德）时，PWM刺激的PBMC培养物几乎只显示总IgM和IgM RF产生增加，这与参与体外对PWM免疫球蛋白反应的放射敏感T抑制细胞一致。在不同培养条件下，9名个体中有5名对PWM和SAC产生了IgM RF，这表明个体产生IgM RF的能力本质上存在于B细胞内。
