Beale M G
Cell Immunol. 1985 May;92(2):427-37. doi: 10.1016/0008-8749(85)90023-1.
Both helper- and suppressor-T-cell activities are generated in the autologous mixed lymphocyte reaction and in pokeweed mitogen (PWM)-stimulated cultures. The addition of low numbers of irradiated non-T cells enhance while high numbers suppress spontaneous and PWM-stimulated IgG synthesis by autologous cells. Monocytes are the principal inducers of suppression and exert their influence within the first 24 hr of culture. Suppression in association with PWM stimulation is nonspecific in nature, T-cell mediated, partially radiosensitive, and resistant to hydrocortisone. Neither indomethacin nor dibutyryl cyclic AMP reverses monocyte-related suppression. These findings suggest that the outcome of in vitro Ig synthesis assays is critically dependent upon monocyte-T-cell interaction.
辅助性T细胞和抑制性T细胞的活性均在自体混合淋巴细胞反应以及美洲商陆丝裂原（PWM）刺激的培养物中产生。添加少量经辐照的非T细胞可增强自体细胞的自发和PWM刺激的IgG合成，而大量添加则会抑制。单核细胞是抑制作用的主要诱导物，并在培养的最初24小时内发挥作用。与PWM刺激相关的抑制本质上是非特异性的，由T细胞介导，部分对辐射敏感，且对氢化可的松有抗性。消炎痛和二丁酰环磷酸腺苷均不能逆转单核细胞相关的抑制作用。这些发现表明，体外Ig合成试验的结果严重依赖于单核细胞与T细胞的相互作用。
