Wang Fenghua, He Enpeng, Li Yanhong, Cai Xiaodi, Ma Wen
a Institute of Physical Education and.
b School of Life Sciences, Xinjiang Normal University , Urumqi , China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Nov;27(6):4093-4094. doi: 10.3109/19401736.2014.1003859. Epub 2015 Jan 28.
In this study, we cloned and sequenced the complete mitochondrial DNA (mtDNA) of Oncorhynchus mykiss triploid to characterize and compare their mitochondrial genomes. The total length of the mitochondrial genome is 16,656 bp with an accession number KP085590. The organization of the mitochondrial genomes was similar to those reported from other fish mitochondrial genomes containing 37 genes (13 protein-coding genes, 2 ribosomal RNA, and 22 transfer RNAs) and a major non-coding control region. Except for ND6 and 8 tRNAs, all other genes are encoded on the heavy strand. The nucleotide skewness for the coding strands of O. mykiss triploid (AT-skew = -0.41, GC-skew = 0.34) is biased toward T and G. The complete mitogenome may provide important date set for the study of genetic mechanism of O. mykiss.
在本研究中，我们克隆并测序了三倍体虹鳟的完整线粒体DNA（mtDNA），以对其线粒体基因组进行表征和比较。线粒体基因组的总长度为16,656 bp，登录号为KP085590。线粒体基因组的组织与其他鱼类线粒体基因组报道的相似，包含37个基因（13个蛋白质编码基因、2个核糖体RNA和22个转运RNA）以及一个主要的非编码控制区。除了ND6和8个tRNA外，所有其他基因都在重链上编码。三倍体虹鳟编码链的核苷酸偏斜度（AT偏斜=-0.41，GC偏斜=0.34）偏向于T和G。完整的线粒体基因组可能为虹鳟遗传机制的研究提供重要的数据集。
