Wang Fenghua, He Enpeng, Li Yanhong, Cai Xiaodi, Ma Wen
a Institute of Physical Education and.
b School of Life Sciences, Xinjiang Normal University , Urumqi , China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Nov;27(6):4153-4154. doi: 10.3109/19401736.2014.1003889. Epub 2015 Jan 29.
In this study, 16 sets of primers were used to amplify contiguous, overlapping segments of the complete mitochondrial DNA (mtDNA) of the hybridized fish (Oncorhynchus mykiss ♀ × Atlantic salmon ♂) in order to characterize and compare their mitochondrial genomes. The total length of the mitochondrial genome is 16,658 bp and deposited in the GenBank with accession numbers KP218514. The organization of the mitochondrial genomes contained 37 genes (13 protein-coding genes, two ribosomal RNA, and 22 transfer RNAs) and a major non-coding control region which was similar to those reported mitochondrial genomes. Most genes were encoded on the H-strand, except for the ND6 and eight tRNA genes, encoding on the L-strand. Similarity and divergence analysis also showed that hybrid offspring were genetically closer to mother parent than father parent. These results indicate that, despite hybridization, the mitochondrial genomes of these hybrids remain maternally inherited.
在本研究中，使用16组引物扩增杂交鱼（虹鳟♀×大西洋鲑♂）完整线粒体DNA（mtDNA）的连续重叠片段，以表征和比较它们的线粒体基因组。线粒体基因组全长16658bp，已存入GenBank，登录号为KP218514。线粒体基因组的组成包含37个基因（13个蛋白质编码基因、2个核糖体RNA和22个转运RNA）以及一个主要的非编码控制区，这与已报道的线粒体基因组相似。除了在轻链上编码的ND6和8个tRNA基因外，大多数基因在重链上编码。相似性和差异分析还表明，杂交后代在遗传上与母本比与父本更接近。这些结果表明，尽管存在杂交，但这些杂种的线粒体基因组仍为母系遗传。
