埃及儿童队列中甲基四氢叶酸还原酶C677T和A1298C多态性与急性淋巴细胞白血病易感性

Methylenetetrahydrofolate reductase C677T and A1298C polymorphism and susceptibility to acute lymphoblastic leukemia in a cohort of Egyptian children.

作者信息

Mosaad Youssef M, Abousamra Nashwa K, Elashery Rasha, Fawzy Iman M, Eldein Omar A Sharaf, Sherief Doaa M, El Azab Hend M M

机构信息

a Clinical Immunology Unit, Clinical Pathology Department and Mansoura Research Center for Cord Stem Cells (MARC_CSC) , Faculty of Medicine, Mansoura University , Mansoura , Egypt.

b Clinical Hematology Unit, Clinical Pathology Department, Faculty of Medicine , Mansoura University , Mansoura , Egypt.

出版信息

Leuk Lymphoma. 2015;56(9):2699-705. doi: 10.3109/10428194.2015.1004170. Epub 2015 Feb 24.

DOI:10.3109/10428194.2015.1004170

PMID:25629981

Abstract

This case-control study was planned to investigate the possible role of methylenetetrahydrofolate reductase (MTHFR) C677T and A1298C polymorphisms as a risk factor for the development of acute lymphoblastic leukemia (ALL) in a cohort of Egyptian children. Typing of MTHFR C677T and A1298C polymorphisms was done using restriction fragment length polymorphism (RFLP) for 100 children with ALL and 100 age- and sex-matched healthy controls. No significant differences were found between patients with ALL and controls for the frequency of MTHFR C677T and A1298C alleles, genotypes, combined genotypes or haplotypes. The C677T and A1298C genotype frequency was different from that in Korean and Chinese populations (p < 0.5) and was similar to that in British, French-Canadian and German-Caucasian populations (p > 0.5). Our findings suggest that MTHFR C677T and A1298C polymorphisms are unlikely to affect the development of childhood ALL in an Egyptian population from Delta.

摘要

本病例对照研究旨在调查亚甲基四氢叶酸还原酶（MTHFR）C677T和A1298C基因多态性作为埃及儿童急性淋巴细胞白血病（ALL）发病风险因素的可能作用。采用限制性片段长度多态性（RFLP）技术对100例ALL患儿及100例年龄和性别匹配的健康对照进行MTHFR C677T和A1298C基因多态性分型。ALL患者与对照在MTHFR C677T和A1298C等位基因、基因型、联合基因型或单倍型频率上未发现显著差异。C677T和A1298C基因型频率与韩国和中国人群不同（p<0.5），与英国、法裔加拿大和德裔高加索人群相似（p>0.5）。我们的研究结果表明，MTHFR C677T和A1298C基因多态性不太可能影响来自三角洲地区的埃及人群儿童ALL的发病。