Wang Chen-Ying, Tsai Sheng-Chueh, Yu Min-Chen, Lin Yu-Fang, Chen Chih-Cheng, Chang Po-Chun
Department of Dentistry, National Taiwan University Hospital, Taipei, Taiwan.
J Periodontol. 2015 May;86(5):674-81. doi: 10.1902/jop.2015.140580. Epub 2015 Jan 29.
This study aims to evaluate the effect of light-emitting diode (LED) light irradiation on the donor wound site of the free gingival graft.
Rat gingival fibroblasts were chosen to assess the cellular activities and in vitro wound healing with 0 to 20 J/cm(2) LED light irradiation. Seventy-two Sprague-Dawley rats received daily 0, 10 (low-dose [LD]), or 20 (high-dose [HD]) J/cm(2) LED light irradiation on the opened palatal wound and were euthanized after 4 to 28 days; the healing pattern was assessed by histology, histochemistry for collagen deposition, and immunohistochemistry for tumor necrosis factor (TNF)-α infiltration. The wound mRNA levels of heme oxygenase-1 (HO-1), TNF-α, the receptor for advanced glycation end products, vascular endothelial growth factor, periostin, Type I collagen, and fibronectin were also evaluated.
Cellular viability and wound closure were significantly promoted, and cytotoxicity was inhibited significantly using 5 J/cm(2) LED light irradiation in vitro. The wound closure, reepithelialization, and collagen deposition were accelerated, and sequestrum formation and inflammatory cell and TNF-α infiltration were significantly reduced in the LD group. HO-1 and TNF-α were significantly upregulated in the HD group, and most of the repair-associated genes were significantly upregulated in both the LD and HD groups at day 7. Persistent RAGE upregulation was noted in both the LD and HD groups until day 14.
LED light irradiation at 660 nm accelerated palatal wound healing, potentially via reducing reactive oxygen species production, facilitating angiogenesis, and promoting provisional matrix and wound reorganization.
本研究旨在评估发光二极管（LED）光照射对游离龈瓣供区创面的影响。
选用大鼠牙龈成纤维细胞，采用0至20 J/cm²的LED光照射来评估细胞活性和体外伤口愈合情况。72只Sprague-Dawley大鼠每天接受0、10（低剂量[LD]）或20（高剂量[HD]）J/cm²的LED光照射腭部开放伤口，4至28天后安乐死；通过组织学、胶原沉积的组织化学以及肿瘤坏死因子（TNF）-α浸润的免疫组织化学评估愈合模式。还评估了血红素加氧酶-1（HO-1）、TNF-α、晚期糖基化终产物受体、血管内皮生长因子、骨膜蛋白、I型胶原和纤连蛋白的伤口mRNA水平。
体外使用5 J/cm²的LED光照射可显著促进细胞活力和伤口闭合，并显著抑制细胞毒性。低剂量组伤口闭合、再上皮化和胶原沉积加速，死骨形成、炎性细胞和TNF-α浸润显著减少。高剂量组HO-1和TNF-α显著上调，在第7天低剂量组和高剂量组中大多数与修复相关的基因均显著上调。低剂量组和高剂量组中晚期糖基化终产物受体持续上调直至第14天。
660 nm的LED光照射可加速腭部伤口愈合，可能是通过减少活性氧生成、促进血管生成以及促进临时基质和伤口重组来实现的。
