Ding Jie, Mellergaard Maiken, Zhu Zhensen, Kwan Peter, Edge Deirdre, Ma Zengshuan, Hebert Lise, Alrobaiea Saad, Iwasaki Takashi, Nielsen Michael Canova Engelbrecht, Tredget Edward E
Wound Healing Research Group, Department of Surgery, Faculty of Medicine and Dentistry, University of Alberta, 161 HMRC, Edmonton, Canada.
Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark.
Open Med (Wars). 2021 Aug 27;16(1):1240-1255. doi: 10.1515/med-2021-0329. eCollection 2021.
Skin grafting is often the only treatment for skin trauma when large areas of tissue are affected. This surgical intervention damages the deeper dermal layers of the skin with implications for wound healing and a risk of scar development. Photobiomodulation (PBM) therapy modulates biological processes in different tissues, with a positive effect on many cell types and pathways essential for wound healing. This study investigated the effect of fluorescent light energy (FLE) therapy, a novel type of PBM, on healing after skin grafting in a dermal fibrotic mouse model. Split-thickness human skin grafts were transplanted onto full-thickness excisional wounds on nude mice. Treated wounds were monitored, and excised xenografts were examined to assess healing and pathophysiological processes essential for developing chronic wounds or scarring. Results demonstrated that FLE treatment initially accelerated re-epithelialization and rete ridge formation, while later reduced neovascularization, collagen deposition, myofibroblast and mast cell accumulation, and connective tissue growth factor expression. While there was no visible difference in gross morphology, we found that FLE treatment promoted a balanced collagen remodeling. Collectively, these findings suggest that FLE has a conceivable effect at balancing healing after skin grafting, which reduces the risk of infections, chronic wound development, and fibrotic scarring.
当大面积组织受到影响时，皮肤移植通常是治疗皮肤创伤的唯一方法。这种手术干预会损害皮肤更深层的真皮层，对伤口愈合有影响，并存在瘢痕形成的风险。光生物调节（PBM）疗法可调节不同组织中的生物过程，对许多对伤口愈合至关重要的细胞类型和途径具有积极作用。本研究调查了一种新型PBM——荧光光能（FLE）疗法对真皮纤维化小鼠模型皮肤移植后愈合的影响。将分层厚度的人皮肤移植物移植到裸鼠的全层切除伤口上。对治疗的伤口进行监测，并检查切除的异种移植物，以评估对慢性伤口或瘢痕形成至关重要的愈合和病理生理过程。结果表明，FLE治疗最初加速了上皮再形成和 rete 嵴形成，而后期减少了新血管形成、胶原沉积、肌成纤维细胞和肥大细胞积累以及结缔组织生长因子表达。虽然大体形态上没有明显差异，但我们发现FLE治疗促进了胶原重塑的平衡。总的来说，这些发现表明FLE在平衡皮肤移植后的愈合方面具有可想象的效果，这降低了感染、慢性伤口发展和纤维化瘢痕形成的风险。
