Jayaraman M, Senthilkumar A, Venkatesalu V
Department of Botany, Annamalai University, Annamalai Nagar, 608 002, Tamil Nadu, India.
Parasitol Res. 2015 Apr;114(4):1511-8. doi: 10.1007/s00436-015-4335-0. Epub 2015 Jan 30.
In the present investigation, larvicidal potential of hexane, choloroform, ethyl acetate, acetone, and methanol extracts of seven aromatic plants, viz., Blumea mollis, Chloroxylon swietenia, Clausena anisata, Feronia limnonia, Lantana camera, Plectranthus amboinicus, and Tagetes erecta were screened against Culex quinquefasciatus, Aedes aegypti, and Anopheles stephensi. The larval mortality was observed after 12 and 24 h of exposure period. The results revealed that all the extracts showed varied levels of larvicidal activity against the mosquito species tested. However, the ethyl acetate extract of Chloroxylon swietenia showed the remarkable larvicidal activity against C. quinquefasciatus, Ae. aegypti, and An. stephensi. After 12 h of exposure period, the larvicidal activity was LC50 = 194.22 and LC90 = 458.83 ppm (C. quinquefasciatus), LC50 = 173.04 and LC90 = 442.73 ppm (Ae. aegypti), and LC50 = 167.28 and LC90 = 433.07 ppm (An. stephensi), and the larvicidal activity after 24-h exposure period was LC50 = 94.12 and LC90 = 249.83 ppm (C. quinquefasciatus), LC50 = 80.58 and LC90 = 200.96 ppm (Ae. aegypti), and LC50 = 76.24 and LC90 = 194.51 ppm (An. stephensi). The larvicidal potential of other plant extracts were in order of ethyl acetate extract of Clausena anisata > methanol extract of P. amboinicus > acetone extract of F. limonia > methanol extract of T. erecta > methanol extract of B. mollis > and methanol extract of L. camera. The results of the present study offer a possible way for further investigations to find out the active molecule responsible for the activity.
在本研究中，对七种芳香植物（即软叶艾纳香、印度乌木、茴香蔻、森林榕、马缨丹、安汶唇形花、万寿菊）的己烷、氯仿、乙酸乙酯、丙酮和甲醇提取物针对致倦库蚊、埃及伊蚊和斯氏按蚊的杀幼虫潜力进行了筛选。在暴露12小时和24小时后观察幼虫死亡率。结果显示，所有提取物对测试的蚊虫种类均表现出不同程度的杀幼虫活性。然而，印度乌木的乙酸乙酯提取物对致倦库蚊、埃及伊蚊和斯氏按蚊表现出显著的杀幼虫活性。暴露12小时后，杀幼虫活性为：LC50 = 194.22且LC90 = 458.83 ppm（致倦库蚊），LC50 = 173.04且LC90 = 442.73 ppm（埃及伊蚊），以及LC50 = 167.28且LC90 = 433.07 ppm（斯氏按蚊）；暴露24小时后的杀幼虫活性为：LC50 = 94.12且LC90 = 249.83 ppm（致倦库蚊），LC50 = 80.58且LC90 = 200.96 ppm（埃及伊蚊），以及LC50 = 76.24且LC90 = 194.51 ppm（斯氏按蚊）。其他植物提取物的杀幼虫潜力顺序为：茴香蔻的乙酸乙酯提取物＞安汶唇形花的甲醇提取物＞森林榕的丙酮提取物＞万寿菊的甲醇提取物＞软叶艾纳香的甲醇提取物＞马缨丹的甲醇提取物。本研究结果为进一步研究找出具有该活性的活性分子提供了一条可能的途径。
