Instituto de Química e Biotecnologia, Universidade Federal de Alagoas, 57072-970, Maceió, Alagoas, Brazil.
Parasitol Res. 2010 Jul;107(2):403-7. doi: 10.1007/s00436-010-1880-4. Epub 2010 May 7.
In an effort to find effective and affordable ways to control of Aedes aegypti L. (Diptera: Culicidae), the larvicidal activities of 94 extracts from ten plant species belonging to eight families [Guettarda grazielae and Spermacoce verticillata (Rubiaceae), Coccoloba mollis and Triplaris americana (Polygonaceae), Eschweilera ovata (Lecytidaceae), Merremia aegyptia (Convolvulaceae), Ouratea nitida (Ochnnaceae), Protium heptaphyllum (Burseraceae), Rourea doniana (Connaraceae), and Tovomita brevistaminea (Guttiferae)], widely found in the northeast of Brazil, were initially screened at 250 microg/mL. Nineteen of the 94 extracts from six plant species (C. mollis, G. grazielae, M. aegyptia, R. doniana, S. verticillata, and T. americana) showed significant activity (>75% mortality) against the fourth instar larvae. Some of these extracts showed 100% mosquito larvae mortality, with LD(50) values lower 200 microg/mL. When compared to synthetic insecticide Temephos (LD(50) 8.7 microg/mL), two of them, hexane (LD(50) 12.1 microg/mL) and ethyl acetate (LD(50) 51.6 microg/mL) extracts from stems of R. doniana and G. grazielae, respectively, were the most effective. The isolation and purification of these extracts are in progress and evaluation of these compounds will be needed to identify the active component.
为了寻找有效且经济实惠的方法来控制埃及伊蚊（双翅目：库蚊科），对来自 10 种植物的 94 种提取物的杀幼虫活性进行了初步筛选，这些植物分属于 8 个科[Guettarda grazielae 和 Spermacoce verticillata（茜草科），Coccoloba mollis 和 Triplaris americana（蓼科），Eschweilera ovata（铁青树科），Merremia aegyptia（旋花科），Ouratea nitida（爵床科），Protium heptaphyllum（金缕梅科），Rourea doniana（使君子科）和 Tovomita brevistaminea（藤黄科）]，这些植物广泛分布于巴西东北部。这些提取物最初在 250μg/mL 时进行筛选，结果显示，来自 6 种植物（C. mollis、G. grazielae、M. aegyptia、R. doniana、S. verticillata 和 T. americana）的 19 种提取物对第四龄幼虫表现出显著的活性（>75%死亡率）。其中一些提取物对蚊虫幼虫的致死率达到 100%，半数致死浓度（LD50）值低于 200μg/mL。与合成杀虫剂 Temephos（LD50 8.7μg/mL）相比，来自 R. doniana 和 G. grazielae 茎的正己烷（LD50 12.1μg/mL）和乙酸乙酯（LD50 51.6μg/mL）提取物是最有效的两种提取物。目前正在对这些提取物进行分离和纯化，并需要对这些化合物进行评估，以确定其活性成分。
