Dubey Lalit Kumar, Moeller Jesper Bonnet, Schlosser Anders, Sorensen Grith Lykke, Holmskov Uffe
Institute of Molecular Medicine, Department of Cancer and Inflammation, University of Southern Denmark, Odense C 5000, Denmark.
Institute of Molecular Medicine, Department of Cancer and Inflammation, University of Southern Denmark, Odense C 5000, Denmark.
Immunobiology. 2015 Jun;220(6):714-21. doi: 10.1016/j.imbio.2015.01.002. Epub 2015 Jan 14.
Aspergillus fumigatus (A. fumigatus) is a ubiquitous fungus that activates, suppresses or modulates the immune response by changing its cell wall structure and by secreting proteases. In this study, we show that chitin acts as an adjuvant in a murine model of A. fumigatus protease induced allergy. The mice were immunised intraperitoneally with A. fumigatus culture filtrate antigen either with or without chitin and were subsequently challenged with the culture filtrate antigen intranasally. Alum was used as an adjuvant control. Compared to alum, chitin induced a weaker inflammatory response in the lungs, measured as the total cell efflux in BAL, EPO and chitinase production. However, chitin enhanced the total IgE, specific IgE and specific IgG1 production as efficiently as alum. Pre-treatment with chitin but not with alum depressed the concentration of the Th2 cytokines IL-4 and IL-13 in BAL fluid. These results shows that chitin, in spite of a reduction of the Th2 cytokine levels in the lungs, enhanced the total and specific IgE production in A. fumigatus culture filtrate induced allergy.
烟曲霉是一种广泛存在的真菌，它通过改变其细胞壁结构和分泌蛋白酶来激活、抑制或调节免疫反应。在本研究中，我们表明几丁质在烟曲霉蛋白酶诱导的小鼠过敏模型中作为佐剂发挥作用。将小鼠腹腔内注射烟曲霉培养滤液抗原，分别添加或不添加几丁质，随后经鼻内给予培养滤液抗原。使用明矾作为佐剂对照。与明矾相比，几丁质诱导的肺部炎症反应较弱，以支气管肺泡灌洗中的总细胞流出、嗜酸性粒细胞过氧化物酶（EPO）和几丁质酶产生来衡量。然而，几丁质与明矾一样有效地增强了总IgE、特异性IgE和特异性IgG1的产生。用几丁质预处理而非明矾预处理可降低支气管肺泡灌洗液中Th2细胞因子IL-4和IL-13的浓度。这些结果表明，尽管几丁质降低了肺部Th2细胞因子水平，但在烟曲霉培养滤液诱导的过敏中增强了总IgE和特异性IgE的产生。
