小鼠中一个假定的睾丸决定基因的染色体定位与表达

Chromosome mapping and expression of a putative testis-determining gene in mouse.

作者信息

Nagamine C M, Chan K M, Kozak C A, Lau Y F

机构信息

Howard Hughes Medical Institute, University of California, San Francisco 94143.

出版信息

Science. 1989 Jan 6;243(4887):80-3. doi: 10.1126/science.2563174.

DOI:10.1126/science.2563174

PMID:2563174

Abstract

Isolation and mapping of a mouse complementary DNA sequence (mouse Y-finger) encoding a multiple, potential zinc-binding, finger protein homologous to the candidate human testis-determining factor gene is reported. Four similar sequences were identified in Hind III-digested mouse genomic DNA. Two (7.2 and 2.0 kb) were mapped to the Y chromosome. Only the 2.0-kb fragment, however, was correlated with testis determination. Polymerase chain reaction analysis suggests both Y loci are transcribed in adult testes. A 3.6-kb fragment was mapped to the X chromosome between the T16H and T6R1 translocation breakpoints, and a fourth (6.0 kb) was mapped to chromosome 10. Hence, mYfin sequences have been duplicated several times in the mouse, although they are not duplicated in humans.

摘要

报道了一种小鼠互补DNA序列（小鼠Y-指蛋白）的分离和定位，该序列编码一种与候选人类睾丸决定因子基因同源的、具有多个潜在锌结合位点的指蛋白。在经Hind III消化的小鼠基因组DNA中鉴定出四个相似序列。其中两个（7.2和2.0 kb）定位于Y染色体。然而，只有2.0 kb的片段与睾丸决定相关。聚合酶链反应分析表明，两个Y位点在成年睾丸中均有转录。一个3.6 kb的片段定位于X染色体上T16H和T6R1易位断点之间，第四个（6.0 kb）定位于10号染色体。因此，mYfin序列在小鼠中已多次重复，尽管在人类中没有重复。