Na Zhi, Song Qi-Shi, Hu Hua-Bin
Nat Prod Commun. 2014 Dec;9(12):1721-2.
A new flavone, 3-methoxy-5-hydroxy-[2",3":7,8] furanoflavone, pubinerone (1), was isolated from the twigs of Millettia pubinervis Kurz, together with ten known flavonoids, karanjin (2), kanjone (3), 3,6-dimethoxy-[2",3":7,8] furanoflavone (4), pongaglabrone (5), pongapin (6), pongaflavone (7), 3,6-dimethoxy- 6",6"-dimethylchromene-[2",3":7,8] flavone (8), pongachromene (9), 3,6-dimethoxy-3',4'-methylenedioxy-6",6"-dimethylchromene-[2",3":7,8] flavone (10) and demethoxykanugin (11). This is the first phytochemical investigation of this plant. The structure of compound 1 was elucidated on the basis of spectroscopic data interpretation, including 1D and 2D NMR and HREIMS analysis. The cytotoxicity of 1 against five human cancer cell lines; HL-60, SMMC-7721, A-549, MCF-7 and SW480, was evaluated, but it was inactive (IC50 > 40 μM).
从柔毛鸡血藤（Millettia pubinervis Kurz）的嫩枝中分离出一种新的黄酮类化合物3-甲氧基-5-羟基-[2",3":7,8]呋喃黄酮，即毛鱼藤酮（1），同时还分离出了十种已知的黄酮类化合物，分别为毛鱼藤素（2）、毛鱼藤醌（3）、3,6-二甲氧基-[2",3":7,8]呋喃黄酮（4）、绒毛润楠黄酮（5）、绒毛润楠素（6）、绒毛润楠黄酮（7）、3,6-二甲氧基-6",6"-二甲基色烯-[2",:7,8]黄酮（8）、绒毛润楠色烯（9）、3,6-二甲氧基-3',4'-亚甲二氧基-6",6"-二甲基色烯-[2",3":7,8]黄酮（10）和去甲氧基鱼藤素（11）。这是对该植物的首次植物化学研究。化合物1的结构通过光谱数据解析得以阐明，包括一维和二维核磁共振及高分辨电子轰击质谱分析。评估了化合物1对五种人类癌细胞系HL-60、SMMC-7721、A-549、MCF-7和SW480的细胞毒性，但该化合物无活性（IC50>40μM）。
