限制内切酶诱导V79、B7中国仓鼠细胞中CAD基因扩增
Induction of CAD gene amplification by restriction endonucleases in V79,B7 Chinese hamster cells.
作者信息
Cavolina P, Agnese C, Maddalena A, Sciandrello G, Di Leonardo A
机构信息
Department of Cell and Developmental Biology A. Monroy, Palermo, Italy.
出版信息
Mutat Res. 1989 Jan-Feb;225(1-2):61-4. doi: 10.1016/0165-7992(89)90034-1.
PMID:2563307
Abstract
The restriction endonucleases PvuII, BamHI and EcoRI were tested for their ability to induce gene amplification in V79,B7 Chinese hamster cells. The results indicate that treatment with these enzymes efficiently increases the frequency of clones resistant to N-phosphonacetyl-L-aspartate, indicating induction of CAD gene amplification.
摘要
对限制性内切酶PvuII、BamHI和EcoRI在V79、B7中国仓鼠细胞中诱导基因扩增的能力进行了测试。结果表明，用这些酶处理可有效提高对N-膦酰乙酰-L-天冬氨酸耐药的克隆频率，表明诱导了CAD基因扩增。
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