Bosch Pablo, Forcato Diego O, Alustiza Fabrisio E, Alessio Ana P, Fili Alejandro E, Olmos Nicotra María F, Liaudat Ana C, Rodríguez Nancy, Talluri Thirumala R, Kues Wilfried A
Departamento de Biología Molecular, Facultad de Ciencias Exactas, Fco-Qcas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Republic of Argentina.
Cell Mol Life Sci. 2015 May;72(10):1907-29. doi: 10.1007/s00018-015-1842-1. Epub 2015 Feb 1.
Transgenic farm animals are attractive alternative mammalian models to rodents for the study of developmental, genetic, reproductive and disease-related biological questions, as well for the production of recombinant proteins, or the assessment of xenotransplants for human patients. Until recently, the ability to generate transgenic farm animals relied on methods of passive transgenesis. In recent years, significant improvements have been made to introduce and apply active techniques of transgenesis and genetic engineering in these species. These new approaches dramatically enhance the ease and speed with which livestock species can be genetically modified, and allow to performing precise genetic modifications. This paper provides a synopsis of enzyme-mediated genetic engineering in livestock species covering the early attempts employing naturally occurring DNA-modifying proteins to recent approaches working with tailored enzymatic systems.
转基因农场动物是用于研究发育、遗传、生殖和疾病相关生物学问题的有吸引力的替代哺乳动物模型，也可用于生产重组蛋白或评估人类患者的异种移植。直到最近，产生转基因农场动物的能力还依赖于被动转基因方法。近年来，在这些物种中引入和应用转基因和基因工程的主动技术取得了重大进展。这些新方法极大地提高了对家畜物种进行基因改造的简便性和速度，并允许进行精确的基因改造。本文概述了家畜物种中酶介导的基因工程，涵盖了从早期使用天然存在的DNA修饰蛋白的尝试到最近使用定制酶系统的方法。
