• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

在对N-（膦酰乙酰基）-L-天冬氨酸耐药的叙利亚仓鼠细胞中rRNA基因与CAD基因的共扩增。

Co-amplification of rRNA genes with CAD genes in N-(phosphonacetyl)-L-aspartate-resistant Syrian hamster cells.

作者信息

Wahl G M, Vitto L, Rubnitz J

出版信息

Mol Cell Biol. 1983 Nov;3(11):2066-75. doi: 10.1128/mcb.3.11.2066-2075.1983.

DOI:10.1128/mcb.3.11.2066-2075.1983
PMID:6318080
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC370073/
Abstract

The amplified CAD genes in N-(phosphonacetyl)-L-aspartate (PALA)-resistant Syrian hamster cells are located in an expanded chromosomal region emanating from the site of the wild-type gene at the tip of the short arm of chromosome B-9. The terminus of B-9 in PALA-sensitive cells contains a cluster of rRNA genes (i.e., a nucleolus organizer, rDNA). We have used a molecular clone containing sequences complementary to Syrian hamster 28S rRNA to investigate whether rDNA is coamplified with CAD genes in the PALA-resistant mutants. In situ hybridization of this probe to metaphase chromosomes demonstrates that rDNA and CAD genes do coamplify in two independently isolated PALA-resistant mutants. The tight linkage of CAD and rDNA genes was demonstrated by their coordinate translocation from B-9 to the end of the long arm of chromosome C-11 in one mutant. Blot hybridization studies substantiate the in situ hybridization results. Both types of analysis indicate that only one or two rDNA genes, on the average, are coamplified per CAD gene. The data are consistent with the model that unequal exchanges between rDNA genes mediate the amplification of CAD genes in the Syrian hamster mutants that were analyzed.

摘要

在对N-（膦酰乙酰基）-L-天冬氨酸（PALA）具有抗性的叙利亚仓鼠细胞中，扩增的CAD基因位于一个扩展的染色体区域，该区域源自B-9染色体短臂末端野生型基因的位点。在对PALA敏感的细胞中，B-9的末端包含一组rRNA基因（即一个核仁组织区，rDNA）。我们使用了一个包含与叙利亚仓鼠28S rRNA互补序列的分子克隆，来研究在PALA抗性突变体中rDNA是否与CAD基因共同扩增。将该探针与中期染色体进行原位杂交表明，在两个独立分离的PALA抗性突变体中，rDNA和CAD基因确实共同扩增。在一个突变体中，CAD和rDNA基因从B-9到C-11染色体长臂末端的协同易位证明了它们的紧密连锁。印迹杂交研究证实了原位杂交结果。两种分析类型均表明，平均每个CAD基因仅共同扩增一两个rDNA基因。这些数据与以下模型一致：在被分析的叙利亚仓鼠突变体中，rDNA基因之间的不等交换介导了CAD基因的扩增。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fb9/370073/ad57e3dadeee/molcellb00111-0189-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fb9/370073/b3376336a0a7/molcellb00111-0186-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fb9/370073/82fe1e02c187/molcellb00111-0187-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fb9/370073/ad57e3dadeee/molcellb00111-0189-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fb9/370073/b3376336a0a7/molcellb00111-0186-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fb9/370073/82fe1e02c187/molcellb00111-0187-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fb9/370073/ad57e3dadeee/molcellb00111-0189-a.jpg

相似文献

1
Co-amplification of rRNA genes with CAD genes in N-(phosphonacetyl)-L-aspartate-resistant Syrian hamster cells.在对N-（膦酰乙酰基）-L-天冬氨酸耐药的叙利亚仓鼠细胞中rRNA基因与CAD基因的共扩增。
Mol Cell Biol. 1983 Nov;3(11):2066-75. doi: 10.1128/mcb.3.11.2066-2075.1983.
2
Properties of single-step mutants of Syrian hamster cell lines resistant to N-(phosphonacetyl)-L-aspartate.对N-（膦酰乙酰基）-L-天冬氨酸具有抗性的叙利亚仓鼠细胞系单步突变体的特性
Mol Cell Biol. 1983 Nov;3(11):2089-98. doi: 10.1128/mcb.3.11.2089-2098.1983.
3
Structure of amplified DNA in different Syrian hamster cell lines resistant to N-(phosphonacetyl)-L-aspartate.对N-（膦酰乙酰基）-L-天冬氨酸有抗性的不同叙利亚仓鼠细胞系中扩增DNA的结构
Mol Cell Biol. 1983 Nov;3(11):2076-88. doi: 10.1128/mcb.3.11.2076-2088.1983.
4
Induction of CAD gene amplification by restriction endonucleases in V79,B7 Chinese hamster cells.限制内切酶诱导V79、B7中国仓鼠细胞中CAD基因扩增
Mutat Res. 1989 Jan-Feb;225(1-2):61-4. doi: 10.1016/0165-7992(89)90034-1.
5
Gene amplification causes overproduction of the first three enzymes of UMP synthesis in N-(phosphonacetyl)-L-aspartate-resistant hamster cells.基因扩增导致对N-（膦酰乙酰基）-L-天冬氨酸耐药的仓鼠细胞中尿苷一磷酸合成的前三种酶过量产生。
J Biol Chem. 1979 Sep 10;254(17):8679-89.
6
The cloning and reintroduction into animal cells of a functional CAD gene, a dominant amplifiable genetic marker.一种功能性CAD基因（一种显性可扩增遗传标记）的克隆及重新导入动物细胞。
Cell. 1981 Dec;27(2 Pt 1):267-77. doi: 10.1016/0092-8674(81)90410-4.
7
Late onset of CAD gene amplification in unamplified PALA resistant Chinese hamster mutants.未扩增的对N-(磷酸乙酰)-L-天冬氨酸（PALA）耐药的中国仓鼠突变体中CAD基因扩增的迟发现象
Cancer Lett. 2000 Mar 31;150(2):119-27. doi: 10.1016/s0304-3835(99)00289-x.
8
Chromosome aberrations associated with CAD gene amplification in Chinese hamster cultured cells.中国仓鼠培养细胞中与CAD基因扩增相关的染色体畸变。
Mutat Res. 1988 May;199(1):111-21. doi: 10.1016/0027-5107(88)90236-9.
9
Multiple mechanisms of N-phosphonacetyl-L-aspartate resistance in human cell lines: carbamyl-P synthetase/aspartate transcarbamylase/dihydro-orotase gene amplification is frequent only when chromosome 2 is rearranged.人类细胞系中对N-膦酰基乙酰-L-天冬氨酸耐药的多种机制：仅当2号染色体发生重排时，氨甲酰磷酸合成酶/天冬氨酸转氨甲酰酶/二氢乳清酸酶基因扩增才较为常见。
Proc Natl Acad Sci U S A. 1997 Mar 4;94(5):1816-21. doi: 10.1073/pnas.94.5.1816.
10
Single-copy and amplified CAD genes in Syrian hamster chromosomes localized by a highly sensitive method for in situ hybridization.通过一种高度灵敏的原位杂交方法定位叙利亚仓鼠染色体中的单拷贝和扩增CAD基因。
Mol Cell Biol. 1982 Mar;2(3):308-19. doi: 10.1128/mcb.2.3.308-319.1982.

引用本文的文献

1
Localization of amplified CAD genes on rearranged chromosomes of Chinese hamster cells.扩增的 CAD 基因在经过重排的中国仓鼠细胞染色体上的定位。
Cytotechnology. 1987 Oct;1(1):25-31. doi: 10.1007/BF00351118.
2
Attenuation of glucocorticoid receptor levels by the H-ras oncogene.H-ras癌基因导致糖皮质激素受体水平降低。
Endocrine. 1995 Apr;3(4):305-12. doi: 10.1007/BF03021410.
3
Inactive ribosomal cistrons are spread throughout the B chromosomes of Rattus rattus (Rodentia, Muridae). Implications for their origin and evolution.

本文引用的文献

1
Satellite DNA in large marker chromosomes of methotrexate-resistant mouse cells.甲氨蝶呤抗性小鼠细胞大标记染色体中的卫星DNA
Cell. 1980 Mar;19(3):709-15. doi: 10.1016/s0092-8674(80)80047-x.
2
Nucleotide sequence and evolution of a mammalian alpha-tubulin messenger RNA.一种哺乳动物α-微管蛋白信使核糖核酸的核苷酸序列与进化
J Mol Biol. 1981 Sep 5;151(1):101-20. doi: 10.1016/0022-2836(81)90223-0.
3
Unequal crossing over in the ribosomal DNA of Saccharomyces cerevisiae.酿酒酵母核糖体DNA中的不等交换
无活性核糖体顺反子分布于黑家鼠（啮齿目，鼠科）的B染色体上。对其起源和进化的启示。
Chromosome Res. 2000;8(4):305-11. doi: 10.1023/a:1009227427575.
4
The relative rDNA content of a NOR determines its level of expression and its probability of becoming active. A sequential silver staining and in-situ hybridization study.核仁组织区（NOR）的相对核糖体DNA（rDNA）含量决定其表达水平及其激活的可能性。一项连续银染和原位杂交研究。
Chromosome Res. 1999;7(7):563-70. doi: 10.1023/a:1009297713973.
5
Recent evolution of NOR-bearing and sex chromosomes of the North African rodent Lemniscomys barbarus.北非啮齿动物巴氏条纹鼠（Lemniscomys barbarus）含核仁组织区（NOR）染色体和性染色体的近期演化
Chromosome Res. 1997 Nov;5(7):481-5. doi: 10.1023/a:1018473131446.
6
Identification of an origin of bidirectional DNA replication in the ubiquitously expressed mammalian CAD gene.在普遍表达的哺乳动物CAD基因中双向DNA复制起点的鉴定。
Mol Cell Biol. 1995 Aug;15(8):4136-48. doi: 10.1128/MCB.15.8.4136.
7
The ribosomal RNA gene cluster in aneuploid chickens: evidence for increased gene dosage and regulation of gene expression.非整倍体鸡中的核糖体RNA基因簇：基因剂量增加及基因表达调控的证据
J Cell Biol. 1985 Nov;101(5 Pt 1):1749-56. doi: 10.1083/jcb.101.5.1749.
8
Amplification of DNA sequences in human multidrug-resistant KB carcinoma cells.人类多药耐药KB癌细胞中DNA序列的扩增
Proc Natl Acad Sci U S A. 1985 Nov;82(22):7661-5. doi: 10.1073/pnas.82.22.7661.
9
Structure of DNA formed in the first step of CAD gene amplification.CAD基因扩增第一步中形成的DNA结构。
EMBO J. 1986 Sep;5(9):2115-21. doi: 10.1002/j.1460-2075.1986.tb04474.x.
10
Similar 150-kilobase DNA sequences are amplified in independently derived methotrexate-resistant Chinese hamster cells.在独立衍生的耐甲氨蝶呤中国仓鼠细胞中扩增出了相似的150千碱基DNA序列。
Mol Cell Biol. 1985 Apr;5(4):619-27. doi: 10.1128/mcb.5.4.619-627.1985.
Nature. 1980 Apr 3;284(5755):426-30. doi: 10.1038/284426a0.
4
Cloned cDNA sequences of the hypoxanthine/guanine phosphoribosyltransferase gene from a mouse neuroblastoma cell line found to have amplified genomic sequences.从小鼠神经母细胞瘤细胞系中克隆出次黄嘌呤/鸟嘌呤磷酸核糖转移酶基因的cDNA序列，该细胞系被发现具有扩增的基因组序列。
Proc Natl Acad Sci U S A. 1982 Mar;79(6):1950-4. doi: 10.1073/pnas.79.6.1950.
5
Evidence for unequal crossing over within the mouse T/t complex.小鼠T/t复合体中不等交换的证据。
Proc Natl Acad Sci U S A. 1980 Oct;77(10):6077-80. doi: 10.1073/pnas.77.10.6077.
6
Triplicated alpha-globin loci in humans.人类中的三重α-珠蛋白基因座。
Proc Natl Acad Sci U S A. 1980 Jan;77(1):518-21. doi: 10.1073/pnas.77.1.518.
7
Spontaneous tandem genetic duplications in Salmonella typhimurium arise by unequal recombination between rRNA (rrn) cistrons.鼠伤寒沙门氏菌中的自发串联基因重复通过rRNA（rrn）顺反子之间的不等交换产生。
Proc Natl Acad Sci U S A. 1981 May;78(5):3113-7. doi: 10.1073/pnas.78.5.3113.
8
Mouse rDNA nontranscribed spacer sequences are found flanking immunoglobulin CH genes and elsewhere throughout the genome.小鼠核糖体DNA非转录间隔序列位于免疫球蛋白重链恒定区基因两侧以及基因组的其他位置。
Cell. 1980 Nov;22(1 Pt 1):179-85. doi: 10.1016/0092-8674(80)90166-x.
9
Patterns of integration of DNA microinjected into cultured mammalian cells: evidence for homologous recombination between injected plasmid DNA molecules.注入培养哺乳动物细胞的DNA整合模式：注入的质粒DNA分子间同源重组的证据
Mol Cell Biol. 1982 Nov;2(11):1372-87. doi: 10.1128/mcb.2.11.1372-1387.1982.
10
Structure of the Syrian hamster ribosomal DNA repeat and identification of homologous and nonhomologous regions shared by human and hamster ribosomal DNAs.叙利亚仓鼠核糖体DNA重复序列的结构以及人类和仓鼠核糖体DNA共享的同源和非同源区域的鉴定。
J Biol Chem. 1982 Jul 10;257(13):7826-33.