Figueiredo David Livingstone Alves, do Amaral Rabello Doralina, Zanette Dalila Luciola, Saggioro Fabiano Pinto, Mamede Rui Celso Martins, Zago Marco Antonio, da Silva Wilson Araújo, Saldanha-Araújo Felipe, Pittella Silva Fabio
Laboratory of Molecular Pathology of Cancer, Faculty of Health Sciences, University of Brasília, Campus Universitário Darcy Ribeiro, Brasília, DF 70.910-900, Brazil.
Center for Cell Therapy and Regional Blood Center, Medical School of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP 14.048-900, Brazil.
Oncol Rep. 2015 Apr;33(4):2017-22. doi: 10.3892/or.2015.3756. Epub 2015 Jan 28.
Larynx cancer is the second most common type of cancer among all head and neck cancers. Deregulation of epigenetic effectors, including altered expression of histone methyltransferases from the MLL (mixed lineage leukemia) family, have been reported in many cancer types, yet little is known concerning their involvement in larynx cancer. Our objective was to determine the expression profile of MLL genes in larynx carcinoma and normal adjacent tissues and correlate this profile to tumor characteristics. We analyzed the expression profile of 5 MLL genes in 13 cases of larynx carcinoma and their adjacent non-tumor tissues using quantitative real-time PCR. MLL3 was significantly downregulated in tumor samples compared to their normal counterparts, and all MLL genes showed decreased expression in advanced tumors compared to tumors in the initial stage. Altered expression in a single MLL gene was associated with a similar alteration in the other MLL genes, revealing a strong correlation of expression in each individual patient. In conclusion, MLL genes may have similar transcriptional control, and decreased expression of these genes may contribute to larynx cancer progression.
喉癌是所有头颈癌中第二常见的癌症类型。在许多癌症类型中都报道了表观遗传效应因子的失调，包括来自MLL（混合谱系白血病）家族的组蛋白甲基转移酶表达改变，但它们在喉癌中的作用却知之甚少。我们的目的是确定MLL基因在喉癌及其相邻正常组织中的表达谱，并将该谱与肿瘤特征相关联。我们使用定量实时PCR分析了13例喉癌及其相邻非肿瘤组织中5个MLL基因的表达谱。与正常对应组织相比，肿瘤样本中MLL3显著下调，并且与早期肿瘤相比，所有MLL基因在晚期肿瘤中的表达均降低。单个MLL基因的表达改变与其他MLL基因的类似改变相关，揭示了每个患者中表达的强相关性。总之，MLL基因可能具有相似的转录调控，这些基因的表达降低可能有助于喉癌进展。
