Hu Jie-Bi, Chen Ting-Ru, Chen Yu-Chie, Urban Pawel L
Department of Applied Chemistry, National Chiao Tung University, 1001 University Rd, Hsinchu, 300, Taiwan.
1] Department of Applied Chemistry, National Chiao Tung University, 1001 University Rd, Hsinchu, 300, Taiwan [2] Institute of Molecular Science, National Chiao Tung University, 1001 University Rd, Hsinchu, 300, Taiwan.
Sci Rep. 2015 Jan 30;5:8135. doi: 10.1038/srep08135.
In order to ascertain optimum conditions for biocatalytic processes carried out in vitro, we have designed a bio-opto-electronic system which ensures real-time compensation for depletion of adenosine triphosphate (ATP) in reactions involving transfer of phosphate groups. The system covers ATP concentration range of 2-48 μM. The report demonstrates feasibility of the device operation using apyrase as the ATP-depleting enzyme.
为了确定体外进行生物催化过程的最佳条件，我们设计了一种生物光电系统，该系统可确保实时补偿涉及磷酸基团转移反应中三磷酸腺苷（ATP）的消耗。该系统涵盖的ATP浓度范围为2 - 48 μM。本报告证明了使用腺苷三磷酸双磷酸酶作为ATP消耗酶时该装置运行的可行性。
