Andexer Jennifer N, Richter Michael
Institute of Pharmaceutical Sciences, University of Freiburg, Albertstrasse 25, 79104 Freiburg (Germany).
Chembiochem. 2015 Feb 9;16(3):380-6. doi: 10.1002/cbic.201402550. Epub 2015 Jan 23.
Adenosine-5'-triphosphate-dependent enzyme catalysed reactions are widespread in nature. Consequently, the enzymes involved have an intrinsic potential for use in syntheses of high value products. Although regeneration systems for ATP starting from adenosine-5'-diphosphate are available, certain limitations exist for both in vitro and in vivo applications requiring ATP regeneration from adenosine-5'-monophosphate, or adenosine. Following a short overview of the chemical and thermodynamic background, this Minireview focuses on emerging enzymes and methodologies for ATP regeneration. A large range of as yet unexploited reactions will be accessible with new, powerful, multistep ATP regeneration systems that use cheap phosphate donors and provide high longevity, compatibility, and robustness under process conditions. Their potential might go far beyond the direct use of ATP in enzymatic reactions; enzyme discovery, and engineering, as well as immobilisation strategies, will help to realise such systems.
依赖三磷酸腺苷（ATP）的酶催化反应在自然界广泛存在。因此，相关酶类具有用于合成高价值产品的内在潜力。虽然存在从二磷酸腺苷（ADP）起始的ATP再生系统，但对于需要从一磷酸腺苷（AMP）或腺苷再生ATP的体外和体内应用而言，仍存在某些限制。在简要概述化学和热力学背景之后，本微型综述聚焦于新兴的ATP再生酶和方法。新型、强大的多步ATP再生系统将能够实现大量尚未开发的反应，这些系统使用廉价的磷酸盐供体，并在工艺条件下具有高寿命、兼容性和稳定性。它们的潜力可能远远超出ATP在酶促反应中的直接应用；酶的发现、工程改造以及固定化策略将有助于实现此类系统。
