Zerargui Fatima, Boumerfeg Sabah, Charef Noureddine, Baghiani Abderrahmane, Djarmouni Meriem, Khennouf Seddik, Arrar Lekhmici, Abu Zarga Musa H, Mubarak Mohammad S
Laboratory of Applied Biochemistry, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif 19000, Algeria and Department of Chemistry, Faculty of Science, The University of Jordan, Amman 11942, Jordan.
Med Chem. 2015;11(5):506-13. doi: 10.2174/1573406411666150130145246.
In this investigation, the screening of two furanocoumarins; 5,8- dimethoxypsoralen (1) and heraclinin (2), isolated from the methanol root-extracts of Tamus communis L for their antioxidant activity and xanthine oxidase inhibitory effect was carried out, using different assays such as DPPH free radical scavenging effect, β- carotene / linoleic acid, xanthine oxidase (XO) inhibition and in addition to blood total antioxidant capacity. Results revealed that the two compounds have significant DPPH radical scavenging activity and effective inhibition of linoleic acid oxidation in a dose-dependent manner; 5,8-dimethoxypsoralen exhibited the highest activity with an I% = 72.69 ± 1.88%. These results indicate that the isolated compounds inhibit xanthine oxidase activity and scavenge superoxide radicals with heraclinin (2) as the more potent xanthine oxidase inhibitor, and 5,8-dimethoxypsoralen (1) as the more effective on cytochrome c reduction, the two tested compounds can effectively protect erythrocytes against hemolytic injury induced by AAPH. These results are promising for further studies of the biological and pathological effects of these natural products.
在本研究中，对从欧白英甲醇根提取物中分离得到的两种呋喃香豆素；5,8-二甲氧基补骨脂素（1）和滨蓟素（2）进行了抗氧化活性和黄嘌呤氧化酶抑制作用的筛选，采用了不同的测定方法，如DPPH自由基清除作用、β-胡萝卜素/亚油酸、黄嘌呤氧化酶（XO）抑制，以及血液总抗氧化能力测定。结果表明，这两种化合物具有显著的DPPH自由基清除活性，并以剂量依赖方式有效抑制亚油酸氧化；5,8-二甲氧基补骨脂素表现出最高活性，I% = 72.69 ± 1.88%。这些结果表明，分离得到的化合物抑制黄嘌呤氧化酶活性并清除超氧阴离子自由基，其中滨蓟素（2）是更有效的黄嘌呤氧化酶抑制剂，而5,8-二甲氧基补骨脂素（1）对细胞色素c还原更有效，这两种受试化合物可有效保护红细胞免受AAPH诱导的溶血损伤。这些结果为进一步研究这些天然产物的生物学和病理学效应提供了希望。
