INVESTIGATION OF ANTIHEMOLYTIC, XANTHINE OXIDASE INHIBITION, ANTIOXIDANT AND ANTIMICROBIAL PROPERTIES OF . AERIAL PART EXTRACTS.

BACKGROUND

In this study, . aerial part extracts (SVEs): were screened for their antihemolytic, xanthine oxidase (XO) inhibition, antioxidant and antimicrobial activities.

MATERIAL AND METHODS

To investigate SVEs antihemolytic activity, the 2,2,-azobis (2-amidinopropane) dihydrochloride (AAPH) was used to induce erythrocyte oxidative hemolysis. In XO inhibition test, xanthine was used as substrate and cytochrome c for generating superoxide anions. The antioxidant activity of SVEs was examined by means of reducing power, DPPH free radical scavenging and iron chelating assays. In addition, SVEs were tested for their antimicrobial effects by evaluating antibacterial and antifungal activities.

RESULTS

Ethyl acetate extract (EAE) contains the highest amount of total polyphenols and flavonoids (661.78 ± 4.00 mg GAE / g E) and (28.81 ± 0.38 mg QE / g E) respectively. In antihemolytic test EAE was the most active extract with an HT value of 165 min. SVEs gave significant inhibitory effects on XO, especially the chloroform extract (ChE) with IC value of 0.0088 ± 0.000 mg/ml. EAE was the most active extract in reducing power essay (EC: 0.0047 ± 0.000 mg/ml) and in DPPH radical scavenging essay (IC: 0.0086 ± 0.000 mg/ml). Finally, the EAE has inhibited the growth of nine bacterial strains with inhibition zone diameters of (12 to 16 mm), but no activities have found against fungal strains.

CONCLUSION

could be considered as a potential source of natural antihemolytic, enzyme modulator, antioxidant and antibacterial agents.