Belkhiri Farida, Baghiani Abderrahmane, Zerroug Mohammed Mihoub, Arrar Lekhmici
Laboratory of Applied Biochemistry, Faculty of Nature and Life Sciences, University of Setifl, Algeria.
Laboratory of Applied Microbiology, Faculty of Nature and Life Sciences, University of Setifl, Algeria.
Afr J Tradit Complement Altern Med. 2017 Jan 13;14(2):273-281. doi: 10.21010/ajtcam.v14i2.29. eCollection 2017.
In this study, . aerial part extracts (SVEs): were screened for their antihemolytic, xanthine oxidase (XO) inhibition, antioxidant and antimicrobial activities.
To investigate SVEs antihemolytic activity, the 2,2,-azobis (2-amidinopropane) dihydrochloride (AAPH) was used to induce erythrocyte oxidative hemolysis. In XO inhibition test, xanthine was used as substrate and cytochrome c for generating superoxide anions. The antioxidant activity of SVEs was examined by means of reducing power, DPPH free radical scavenging and iron chelating assays. In addition, SVEs were tested for their antimicrobial effects by evaluating antibacterial and antifungal activities.
Ethyl acetate extract (EAE) contains the highest amount of total polyphenols and flavonoids (661.78 ± 4.00 mg GAE / g E) and (28.81 ± 0.38 mg QE / g E) respectively. In antihemolytic test EAE was the most active extract with an HT value of 165 min. SVEs gave significant inhibitory effects on XO, especially the chloroform extract (ChE) with IC value of 0.0088 ± 0.000 mg/ml. EAE was the most active extract in reducing power essay (EC: 0.0047 ± 0.000 mg/ml) and in DPPH radical scavenging essay (IC: 0.0086 ± 0.000 mg/ml). Finally, the EAE has inhibited the growth of nine bacterial strains with inhibition zone diameters of (12 to 16 mm), but no activities have found against fungal strains.
could be considered as a potential source of natural antihemolytic, enzyme modulator, antioxidant and antibacterial agents.
在本研究中，对地上部分提取物（SVEs）进行了抗溶血、黄嘌呤氧化酶（XO）抑制、抗氧化和抗菌活性筛选。
为研究SVEs的抗溶血活性，使用2,2-偶氮二（2-脒基丙烷）二盐酸盐（AAPH）诱导红细胞氧化溶血。在XO抑制试验中，以黄嘌呤为底物，细胞色素c用于产生超氧阴离子。通过还原力、DPPH自由基清除和铁螯合试验检测SVEs的抗氧化活性。此外，通过评估抗菌和抗真菌活性来测试SVEs的抗菌效果。
乙酸乙酯提取物（EAE）分别含有最高含量的总多酚和黄酮类化合物（661.78±4.00 mg GAE/g E）和（28.81±0.38 mg QE/g E）。在抗溶血试验中，EAE是活性最高的提取物，HT值为165分钟。SVEs对XO有显著抑制作用，尤其是氯仿提取物（ChE），IC值为0.0088±0.000 mg/ml。EAE在还原力试验（EC：0.0047±0.000 mg/ml）和DPPH自由基清除试验（IC：0.0086±0.000 mg/ml）中是活性最高的提取物。最后，EAE抑制了9种细菌菌株的生长，抑菌圈直径为（12至16 mm），但未发现对真菌菌株有活性。
可被视为天然抗溶血剂、酶调节剂、抗氧化剂和抗菌剂的潜在来源。
