Wenz Lena-Sophie, Opaliński Łukasz, Wiedemann Nils, Becker Thomas
Institut für Biochemie und Molekularbiologie, ZBMZ, Universität Freiburg, 79104 Freiburg, Germany.
Institut für Biochemie und Molekularbiologie, ZBMZ, Universität Freiburg, 79104 Freiburg, Germany; BIOSS Centre for Biological Signalling Studies, Universität Freiburg, 79104 Freiburg, Germany.
Biochim Biophys Acta. 2015 May;1853(5):1119-29. doi: 10.1016/j.bbamcr.2015.01.012. Epub 2015 Jan 26.
The function of mitochondria depends on the import of proteins, which are synthesized as precursors on cytosolic ribosomes. The majority of the precursor proteins are sorted into the mitochondrial subcompartments via five distinct routes. Recent studies revealed that molecular cooperation between protein machineries is a central feature of mitochondrial protein biogenesis. First, coupling to various partner proteins affects the substrate specificity of translocases and single translocation steps. Second, there is a substantial cooperation between different protein translocases in the import of specific precursor proteins. Third, protein transport is intimately linked to processing, folding and assembly reactions. Fourth, sorting of precursor proteins is functionally and physically connected to protein machineries, which fulfill central functions for respiration, maintenance of membrane architecture and form contacts to the endoplasmic reticulum. Therefore, we propose that the protein transport systems are part of a complicated protein network for mitochondrial biogenesis.
线粒体的功能依赖于蛋白质的输入，这些蛋白质在胞质核糖体上以前体形式合成。大多数前体蛋白通过五条不同的途径被分选到线粒体亚区室中。最近的研究表明，蛋白质机器之间的分子协作是线粒体蛋白质生物合成的核心特征。首先，与各种伴侣蛋白的偶联会影响转运体的底物特异性和单个转运步骤。其次，在特定前体蛋白的输入过程中，不同的蛋白质转运体之间存在大量协作。第三，蛋白质运输与加工、折叠和组装反应密切相关。第四，前体蛋白的分选在功能和物理上与蛋白质机器相连，这些蛋白质机器对呼吸作用、维持膜结构以及与内质网形成接触起着核心作用。因此，我们提出蛋白质运输系统是线粒体生物合成复杂蛋白质网络的一部分。
