Zhao Yang, Chen Deyong, Luo Yana, Chen Feng, Zhao Xiaoting, Jiang Mei, Yue Wentao, Long Rong, Wang Junbo, Chen Jian
State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China.
Department of Cellular and Molecular Biology, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China.
Sensors (Basel). 2015 Jan 27;15(2):2763-73. doi: 10.3390/s150202763.
This paper presents a microfluidics-based approach capable of continuously characterizing instantaneous Young's modulus (E(instantaneous)) and specific membrane capacitance (C(specific membrane)) of suspended single cells. In this method, cells were aspirated through a constriction channel while the cellular entry process into the constriction channel was recorded using a high speed camera and the impedance profiles at two frequencies (1 kHz and 100 kHz) were simultaneously measured by a lock-in amplifier. Numerical simulations were conducted to model cellular entry process into the constriction channel, focusing on two key parameters: instantaneous aspiration length (L(instantaneous)) and transitional aspiration length (L(transitional)), which was further translated to E(instantaneous). An equivalent distribution circuit model for a cell travelling in the constriction channel was used to determine C(specific membrane). A non-small-cell lung cancer cell line 95C (n = 354) was used to evaluate this technique, producing E(instantaneous) of 2.96 ± 0.40 kPa and Cspecific membrane of 1.59 ± 0.28 μF/cm2. As a platform for continuous and simultaneous characterization of cellular E(instantaneous) and C(specific membrane), this approach can facilitate a more comprehensive understanding of cellular biophysical properties.
本文提出了一种基于微流控的方法，该方法能够连续表征悬浮单细胞的瞬时杨氏模量（E（瞬时））和比膜电容（C（比膜））。在该方法中，细胞通过收缩通道被抽吸，同时使用高速相机记录细胞进入收缩通道的过程，并通过锁相放大器同时测量两个频率（1 kHz和100 kHz）下的阻抗谱。进行了数值模拟以对细胞进入收缩通道的过程进行建模，重点关注两个关键参数：瞬时抽吸长度（L（瞬时））和过渡抽吸长度（L（过渡）），后者进一步转换为E（瞬时）。使用一个用于细胞在收缩通道中移动的等效分布电路模型来确定C（比膜）。使用非小细胞肺癌细胞系95C（n = 354）来评估该技术，得出E（瞬时）为2.96±0.40 kPa，C（比膜）为1.59±0.28 μF/cm²。作为一种用于连续同时表征细胞E（瞬时）和C（比膜）的平台，该方法有助于更全面地了解细胞的生物物理特性。
