Mastronicola R, Berteau C, Tu Q, Cortese S, Guillet J, Phulpin B, Gangloff P, Bezdetnaya L, Merlin J-L, Faure G, Dolivet G
Université de Lorraine, CRAN, UMR 7039, 54506, Vandœuvre-Lès-Nancy, France.
CNRS, CRAN, UMR 7039, 54506, Vandœuvre-Lès-Nancy, France.
Eur Arch Otorhinolaryngol. 2016 Feb;273(2):465-9. doi: 10.1007/s00405-015-3522-z. Epub 2015 Jan 30.
The dissemination of individual tumor cells is a common phenomenon in solid cancers. Detection of tumor cells in bone marrow disseminated tumor cells (DTC) and in peripheral blood circulating tumor cells (CTC) in nonmetastatic situation is of high prognostic significance. Compared to breast, colon and prostate cancers, the studies on CTC and DTC in head and neck cancers are sparse. The objective of our study was to detect DTC in drains after neck dissection. Fourteen patients undergoing surgery for stages III and IV head and neck cancers were enrolled in this study--twelve presenting with squamous cell carcinoma and two with adenocarcinoma. Redon drain analysis was performed by the Cellsearch method using immunomagnetic and fluorescence approaches. A positivity threshold value was set at 2DTC/7.5 ml of the sample. Tumor cells were detected in drains of 69 % of patients a few days after surgery. The range of quantification was 3-2,094 DTC/5 ml and we showed morphological differences between the two types of carcinoma cells. DTC were detected after neck dissection both in squamous cell carcinoma and in adenocarcinoma. Potential clinical significance of tumor cells needs to be further investigated as their presence could affect pre-surgical and post-operative treatments.
单个肿瘤细胞的播散是实体癌中的常见现象。在非转移情况下检测骨髓中播散肿瘤细胞（DTC）和外周血中循环肿瘤细胞（CTC）中的肿瘤细胞具有很高的预后意义。与乳腺癌、结肠癌和前列腺癌相比，头颈部癌中关于CTC和DTC的研究较少。我们研究的目的是检测颈部清扫术后引流液中的DTC。本研究纳入了14例接受III期和IV期头颈部癌手术的患者，其中12例为鳞状细胞癌，2例为腺癌。通过Cellsearch方法采用免疫磁珠和荧光方法进行Redon引流液分析。将阳性阈值设定为每7.5毫升样本中有2个DTC。术后数天，在69%的患者引流液中检测到肿瘤细胞。定量范围为每5毫升3 - 2094个DTC，并且我们显示了两种癌细胞之间的形态差异。在鳞状细胞癌和腺癌的颈部清扫术后均检测到了DTC。肿瘤细胞的潜在临床意义需要进一步研究，因为它们的存在可能会影响术前和术后治疗。
