Weissig Volkmar
Department of Pharmaceutical Sciences, College of Pharmacy Glendale, Midwestern University, Glendale Hall 236-18, 19555 N 59th Avenue, Glendale, AZ, 85308, USA,
Methods Mol Biol. 2015;1265:1-11. doi: 10.1007/978-1-4939-2288-8_1.
DQAsomes (dequalinium-based liposome-like vesicles) are the prototype for all mitochondria-targeted vesicular pharmaceutical nanocarrier systems. First described in 1998, they have been successfully explored for the delivery of DNA and low-molecular weight molecules to mitochondria within living mammalian cells. Potential areas of application involve mitochondrial gene therapy, antioxidant therapy as well as apoptosis-based anticancer chemotherapy. Here, detailed protocols for the preparation, characterization, and application of DQAsomes are given.
双喹嗡脂质体（基于双喹嗡的类脂质体囊泡）是所有靶向线粒体的囊泡药物纳米载体系统的原型。它们于1998年首次被描述，已成功用于将DNA和低分子量分子递送至活的哺乳动物细胞内的线粒体。潜在的应用领域包括线粒体基因治疗、抗氧化治疗以及基于细胞凋亡的抗癌化疗。本文给出了双喹嗡脂质体制备、表征及应用的详细方案。
