Sahoo Tapasa Kumar, Jena Prasant Kumar, Nagar Nidhi, Patel Amiya Kumar, Seshadri Sriram
School of Life Sciences, Sambalpur University, Jyotivihar, Burla, Sambalpur, 768019, Odisha, India.
Probiotics Antimicrob Proteins. 2015 Jun;7(2):126-36. doi: 10.1007/s12602-015-9184-8.
We report the evaluation of probiotic properties of potent lactic acid bacteria (LAB) from the gut of freshwater fishes, Labeo rohita and Catla catla, for eventually developing probiotic strains for the prevention of bacterial infections in aquaculture and food preservation. Five different LAB strains were isolated and characterized for their probiotic properties. Based on physiological, morphological and biochemical characteristics, three isolates from Labeo rohita and two from Catla catla were identified as putative probiotics and were denoted as LR11, LR14 and LR16 and CC3 and CC4, respectively. Isolates CC3 and CC4 were acid (pH 2.5) and bile salt (0.3% oxygall) tolerant and exhibited strong antibacterial activities against all pathogens including Aeromonas hydrophila. In addition, all LAB isolates were susceptible to tested antibiotics, except CC3 and CC4 which were vancomycin resistant. Furthermore, the isolates CC3 and CC4 showed significantly higher in vitro cell surface properties, i.e., hydrophobicity, auto- and co-aggregation. Biochemical tests, PCR detection and 16S rRNA sequence analysis established that LR11, LR14, LR16, CC3 and CC4 are Enterococcus avium TSU11, Enterococcus pseudoavium TSU14, Enterococcus raffinosus TSU16, Lactobacillus gasseri TSU3 and Lactobacillus animalis TSU4, respectively. Studies revealed that, Lactobacillus gasseri TSU3 and Lactobacillus animalis TSU4 are ideal probiotic candidates for its use in aquaculture and require further exploratory in vivo evaluation and safety studies.
我们报告了对来自淡水鱼（印度野鲮和印度鲶）肠道的强效乳酸菌（LAB）的益生菌特性进行的评估，目的是最终开发出用于预防水产养殖中细菌感染和食品保鲜的益生菌菌株。分离出五种不同的LAB菌株，并对其益生菌特性进行了表征。根据生理、形态和生化特征，从印度野鲮中分离出的三株菌株和从印度鲶中分离出的两株菌株被鉴定为推定益生菌，分别命名为LR11、LR14和LR16以及CC3和CC4。分离株CC3和CC4耐酸（pH 2.5）和耐胆盐（0.3%氧胆酸），并对包括嗜水气单胞菌在内的所有病原体表现出强大的抗菌活性。此外，所有LAB分离株对测试抗生素敏感，但CC3和CC4对万古霉素耐药。此外，分离株CC3和CC4表现出显著更高的体外细胞表面特性，即疏水性、自聚集和共聚集性。生化试验、PCR检测和16S rRNA序列分析确定，LR11、LR14、LR16、CC3和CC4分别为鸟肠球菌TSU11、伪鸟肠球菌TSU14、棉籽糖肠球菌TSU16、加氏乳杆菌TSU3和动物乳杆菌TSU4。研究表明，加氏乳杆菌TSU3和动物乳杆菌TSU4是水产养殖中理想的益生菌候选菌株，需要进一步进行体内探索性评估和安全性研究。
